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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • FC experiment of human PBMCs using 98143-2-RR (same clone as 98143-2-PBS)

    FC experiment of human PBMCs using 98143-2-RR (same clone as 98143-2-PBS)

    1x10^6 untreated or PHA-treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human Ki-67 Rabbit Recombinant Antibody (98143-2-RR, Clone: 241499B1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 98143-2-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98143-2-RR (same clone as 98143-2-PBS)

    FC experiment of human PBMCs using 98143-2-RR (same clone as 98143-2-PBS)

    1x10^6 PHA-treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human Ki-67 Rabbit Recombinant Antibody (98143-2-RR, Clone:241499B1) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were intracellularly stained with 0.25 ug Anti-Human Ki-67 Rabbit Recombinant Antibody (98143-2-RR, Clone: 241499B1) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 98143-2-PBS in a different storage buffer formulation.

Anti-Human Ki-67 Rabbit Recombinant Antibody, PBS Only

Ki-67 Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. 98143-2-PBS

Print datasheet

CloneNo. 241499B1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

MKI67, KI67, Ki 67, Antigen KI-67, Antigen identified by monoclonal antibody Ki-67

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy KI67 Ready-To-Use IHC Kit. KI67 primary antibody included.

产品信息

98143-2-PBS targets Ki-67 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name antigen identified by monoclonal antibody Ki-67
Synonyms MKI67, KI67, Ki 67, Antigen KI-67, Antigen identified by monoclonal antibody Ki-67
Calculated Molecular Weight 359 kDa
GenBank Accession NumberNM_002417
Gene Symbol KI67
Gene ID (NCBI) 4288
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP46013
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.