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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
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验证数据展示

  • FC experiment of human PBMCs using 98143-2-RR (same clone as 98143-2-PBS)

    FC experiment of human PBMCs using 98143-2-RR (same clone as 98143-2-PBS)

    1x10^6 untreated or PHA-treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human Ki-67 Rabbit Recombinant Antibody (98143-2-RR, Clone: 241499B1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 98143-2-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 98143-2-RR (same clone as 98143-2-PBS)

    FC experiment of human PBMCs using 98143-2-RR (same clone as 98143-2-PBS)

    1x10^6 PHA-treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human Ki-67 Rabbit Recombinant Antibody (98143-2-RR, Clone:241499B1) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were intracellularly stained with 0.25 ug Anti-Human Ki-67 Rabbit Recombinant Antibody (98143-2-RR, Clone: 241499B1) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 98143-2-PBS in a different storage buffer formulation.

Anti-Human Ki-67 Rabbit Recombinant Antibody, PBS Only

Ki-67 Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. 98143-2-PBS

产品说明书

CloneNo. 241499B1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

MKI67, KI67, Ki 67, Antigen KI-67, Antigen identified by monoclonal antibody Ki-67

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy KI67 Ready-To-Use IHC Kit. KI67 primary antibody included.

产品信息

98143-2-PBS targets Ki-67 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 antigen identified by monoclonal antibody Ki-67
别名 MKI67, KI67, Ki 67, Antigen KI-67, Antigen identified by monoclonal antibody Ki-67
计算分子量 359 kDa
GenBank蛋白编号NM_002417
基因名称 KI67
Gene ID (NCBI) 4288
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP46013
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.