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验证数据展示

  • FC experiment of human PBMCs using 98143-2-RR

    FC experiment of human PBMCs using 98143-2-RR

    1x10^6 untreated or PHA-treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human Ki-67 Rabbit Recombinant Antibody (98143-2-RR, Clone: 241499B1) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • FC experiment of human PBMCs using 98143-2-RR

    FC experiment of human PBMCs using 98143-2-RR

    1x10^6 PHA-treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human Ki-67 Rabbit Recombinant Antibody (98143-2-RR, Clone:241499B1) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were intracellularly stained with 0.25 ug Anti-Human Ki-67 Rabbit Recombinant Antibody (98143-2-RR, Clone: 241499B1) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Anti-Human Ki-67 Rabbit Recombinant Antibody

Ki-67 Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. 98143-2-RR

产品说明书

CloneNo. 241499B1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

MKI67, KI67, Ki 67, Antigen KI-67, Antigen identified by monoclonal antibody Ki-67

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inPHA treated human PBMCs
Planning an IHC experiment? We recommend our IHCeasy KI67 Ready-To-Use IHC Kit. KI67 primary antibody included.

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98143-2-RR targets Ki-67 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 antigen identified by monoclonal antibody Ki-67
别名 MKI67, KI67, Ki 67, Antigen KI-67, Antigen identified by monoclonal antibody Ki-67
计算分子量 359 kDa
GenBank蛋白编号NM_002417
基因名称 KI67
Gene ID (NCBI) 4288
RRIDAB_3672289
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP46013
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.

实验方案

Product Specific Protocols
FC protocol for Ki-67 antibody 98143-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols