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Anti-Human CD16b Rabbit Recombinant Antibody

CD16b Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241713E3

Cat no : 98192-1-RR

Print datasheet

Synonyms

FCGR3B, FCG3, Fc gamma RIIIb, Fc gamma RIII beta, Fc gamma RIII

验证数据展示

  • FC experiment of human peripheral blood leukocytes using 98192-1-RR

    FC experiment of human peripheral blood leukocytes using 98192-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16b Rabbit Recombinant Antibody (98192-1-RR, Clone:241713E3) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

  • FC experiment of human peripheral blood leukocytes using 98192-1-RR

    FC experiment of human peripheral blood leukocytes using 98192-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD16b Rabbit Recombinant Antibody (98192-1-RR, Clone:241713E3) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98192-1-RR targets CD16b in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Fc fragment of IgG, low affinity IIIb, receptor (CD16b)
Synonyms FCGR3B, FCG3, Fc gamma RIIIb, Fc gamma RIII beta, Fc gamma RIII
Gene Symbol FCGR3B
Gene ID (NCBI) 2215
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD16 is a low affinity Fc receptor. It has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b), encoded by two nearly identical genes, FCGR3A and FCGR3B. In humans, FcγRIIIa/CD16a is a type I transmembrane receptor expressed on macrophages and NK cells. FcγRIIIb/CD16b is a GPI-linked Fc-receptor highly expressed on neutrophils. The two proteins differ by only four amino acid residues in the mature proteins (PMID: 30361439).

实验方案

Product Specific Protocols
FC protocol for CD16b antibody 98192-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols