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CD73 Recombinant antibody

CD73 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

241541G9

Cat no : 98199-1-RR

Print datasheet

Synonyms

Ecto-5'-nucleotidase, EC:3.1.3.99, 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase

验证数据展示

  • FC experiment of mouse splenocytes using 98199-1-RR

    FC experiment of mouse splenocytes using 98199-1-RR

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD73 Rabbit Recombinant Antibody (98199-1-RR, Clone: 241541G9) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Mouse CD3. Cells were not fixed.

  • FC experiment of mouse splenocytes using 98199-1-RR

    FC experiment of mouse splenocytes using 98199-1-RR

    1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD73 Rabbit Recombinant Antibody (98199-1-RR, Clone: 241541G9) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • Affinity and Kinetic Characterization of 98199-1-RR

    Affinity and Kinetic Characterization of 98199-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98199-1-RR against Mouse CD73 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive FC detected inmouse splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98199-1-RR targets CD73 in FC applications and shows reactivity with mouse samples.

Tested Applications FC Application Description
Tested Reactivity mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name 5' nucleotidase, ecto
Synonyms Ecto-5'-nucleotidase, EC:3.1.3.99, 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase
Calculated Molecular Weight64kDa
GenBank Accession NumberNM_011851.4
Gene Symbol Nt5e
Gene ID (NCBI) 23959
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ61503
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

实验方案

Product Specific Protocols
FC protocol for CD73 antibody 98199-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols