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验证数据展示

  • FC experiment of human PBMCs using 98203-1-RR

    FC experiment of human PBMCs using 98203-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human IL-4RA/CD124 Rabbit Recombinant Antibody (98203-1-RR, Clone:241824D8) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 98203-1-RR

    FC experiment of human PBMCs using 98203-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human IL-4RA/CD124 Rabbit Recombinant Antibody (98203-1-RR, Clone:241824D8) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

  • Affinity and Kinetic Characterization of 98203-1-RR

    Affinity and Kinetic Characterization of 98203-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98203-1-RR against Human IL-4RA/CD124 were performed. The affinity constant is 53.6 pM.

Anti-Human IL-4RA/CD124 Rabbit Recombinant Antibody

IL-4RA/CD124 Recombinant Antibody for FC
Cat No. 98203-1-RR

产品说明书

CloneNo. 241824D8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

IL4R, IL 4R subunit alpha, IL 4R alpha, IL 4 receptor subunit alpha, IL 4 binding protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98203-1-RR targets IL-4RA/CD124 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interleukin 4 receptor
别名 IL4R, IL 4R subunit alpha, IL 4R alpha, IL 4 receptor subunit alpha, IL 4 binding protein
计算分子量90 kDa
GenBank蛋白编号NM_000418.4
基因名称 IL-4R
Gene ID (NCBI) 3566
RRIDAB_3672335
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP24394-1
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

IL-4 receptor alpha chain (IL-4RA), also known as CD124, is a transmembrane glycoprotein expressed on a wide variety of hematopoietic and non-hematopoietic cells. It is a receptor for both IL-4 and IL-13. IL-4RA can complex with the common gamma chain (IL-2R gamma or CD132) and utilizes JAK1 and JAK2 for signal transduction, while complexes of IL-4RA with IL-13RA1 subunit mediate signals via JAK2 and Tyk2. The IL-4 response is involved in promoting Th2 differentiation. The IL-4/IL-13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation.

实验方案

Product Specific Protocols
FC protocol for IL-4RA/CD124 antibody 98203-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols