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Anti-Human IL-7 Rabbit Recombinant Antibody

IL-7 Uni-rAbTM Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

241000F3

Cat no : 98206-1-RR

Print datasheet

Synonyms

IL7, Interleukin-7, interleukin 7, IL 7, 241000F3

验证数据展示

  • FC experiment of Transfected HEK-293T using 98206-1-RR

    FC experiment of Transfected HEK-293T using 98206-1-RR

    1x10^6 HEK-293T cells or IL-7 transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Human IL-7 Rabbit Recombinant Antibody (98206-1-RR, Clone:241000F3) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of Transfected HEK-293T using 98206-1-RR

    FC experiment of Transfected HEK-293T using 98206-1-RR

    1x10^6 HEK-293T cells (left) or IL-7 transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Human IL-7 Rabbit Recombinant Antibody (98206-1-RR, Clone:241000F3) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 98206-1-RR

    Affinity and Kinetic Characterization of 98206-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98206-1-RR against Human IL-7 were performed. The affinity constant is 8.55 nM.

经过测试的应用

Positive FC (Intra) detected inTransfected HEK-293T cells

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98206-1-RR targets IL-7 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 7
Synonyms IL7, Interleukin-7, interleukin 7, IL 7, 241000F3
Calculated Molecular Weight20 kDa
GenBank Accession NumberNM_000880.4
Gene Symbol IL-7
Gene ID (NCBI) 3574
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP13232-1
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

Interleukin-7 (IL-7) is a cytokine involved in B and T cell development. It plays an active role in the development, survival, maintaining and restoring homeostasis of mature T lymphocytes and is a key regulator of the commitment, survival, proliferation and maturation of B cells during development. Furthermore, IL-7 can improve the antiviral function and expansion of natural killer (NK) cells and regulate the development and differentiation of dendritic cells. IL-7 has also been reported as a regulator of the development of central nervous system and myogenesis and skeletal muscle cell migration. (PMID: 29663382; 29655570; 29449560)

实验方案

Product Specific Protocols
FC protocol for IL-7 antibody 98206-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols