FITC-conjugated ATF4 Polyclonal antibody
ATF4 Polyclonal Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF/ICC
Conjugate
FITC Fluorescent Dye
验证数据展示
经过测试的应用
Positive IF detected in | HepG2 cells, Tunicamycin treated HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy ATF4 Ready-To-Use IHC Kit. ATF4 primary antibody included.
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 1 publications below |
产品信息
FITC-10835 targets ATF4 in IF applications and shows reactivity with human, mouse samples.
Tested Applications | IF/ICC Application Description |
Cited Applications | IF |
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Immunogen | ATF4 fusion protein Ag1279 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | activating transcription factor 4 (tax-responsive enhancer element B67) |
Synonyms | ATF4, CREB 2, CREB2, DNA binding protein TAXREB67, FITC-10835, TAXREB67, TXREB |
Calculated Molecular Weight | 39 kDa |
Observed Molecular Weight | 45-50 kDa |
GenBank Accession Number | BC022088 |
Gene Symbol | ATF4 |
Gene ID (NCBI) | 468 |
RRID | AB_2883737 |
Conjugate | FITC Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 498 nm / 526 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P18848 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATF4 is a transcription factor, that accumulates predominantly in osteoblasts, where it regulates terminal osteoblast differentiation and bone formation[PMID: 19016586]. As a basic leucine-zipper (bZip) transcription factor, ATF4 can regulate amino acid metabolism, cellular redox state, and anti-stress responses. It also regulates age-related and diet-induced obesity and glucose homeostasis in mammals, and has conserved metabolic functions in flies[PMID: 19726872]. Due to its location at chromosome 22q13, a region linked to schizophrenia, ATF4 is considered as a positional candidate gene for schizophrenia[PMID: 18163433]. Otherwise, since ATF4 is induced by tumour microenvironmental factors, and regulates processes relevant to cancer progression, it might serve as a potential therapeutic target in cancer. Endogenous ATF4 protein has a molecular mass of 50kd. [PMID: 17726049]. This antibody is a rabbit polyclonal antibody raised against full length human ATF4 antigen. The antibody recognizes the 38kd ATF4 protein and its phosphorylated forms (50kd). ATF4 can bind DNA as a homodimer and as a heterodimer. ATF4 is ubiquitinated by SCF(BTRC) in response to mTORC1 signal, followed by proteasomal degradation and leading to down-regulate expression of SIRT4, so the molecular weight of ATF4 may be 70 kDa.
实验方案
Product Specific Protocols | |
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IF protocol for FITC ATF4 antibody FITC-10835 | Download protocol |
FC protocol for FITC ATF4 antibody FITC-10835 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cancer Cell Int MiR-876-5p modulates head and neck squamous cell carcinoma metastasis and invasion by targeting vimentin. |