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ASGR1 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human, mouse, rat

Applications

Cytometric bead array, Sandwich ELISA

Range

Cytometric Bead Array: 0.625-80 ng/mL

Sandwich ELISA: 7.8-1000 pg/mL

Cat No : MP00681-3

Print datasheet

Synonyms

ASGP R 1, ASGPR, ASGPR 1, ASGR1, asialoglycoprotein receptor 1, CLEC4H1, Hepatic lectin H1, HL 1, Hs.12056

Validation Data Gallery

  • Sandwich ELISA standard curve of MP00681-3, Human ASGR1 Recombinant Matched Antibody Pair - PBS only. 83691-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0946. 83691-3-PBS was HRP conjugated as the detection antibody. Range: 7.8-1000 pg/mL.

    Sandwich ELISA standard curve of MP00681-3

    Sandwich ELISA standard curve of MP00681-3, Human ASGR1 Recombinant Matched Antibody Pair - PBS only. 83691-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0946. 83691-3-PBS was HRP conjugated as the detection antibody. Range: 7.8-1000 pg/mL.

  • Serum of sixteen individual healthy human donors was measured. The ASGR1 concentration of detected samples was determined to be 1,563.4 pg/mL with a range of 450.5-3,950.7 pg/mL.

    Sample test of MP00681-3

    Serum of sixteen individual healthy human donors was measured. The ASGR1 concentration of detected samples was determined to be 1,563.4 pg/mL with a range of 450.5-3,950.7 pg/mL.

  • The mean human ASGR1 concentration was determined to be 175.6 ng/mL in HepG2 cell extract based on a 3.7 mg/mL extract load.

    Sample test of MP00681-3

    The mean human ASGR1 concentration was determined to be 175.6 ng/mL in HepG2 cell extract based on a 3.7 mg/mL extract load.

  • Cytometric bead array standard curve of MP00681-3, ASGR1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83691-1-PBS. Detection antibody: 83691-3-PBS. Standard: Eg0946. Range: 0.625-80 ng/mL.

    Cytometric bead array standard curve of MP00681-3

    Cytometric bead array standard curve of MP00681-3, ASGR1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83691-1-PBS. Detection antibody: 83691-3-PBS. Standard: Eg0946. Range: 0.625-80 ng/mL.

Tested Applications

Cytometric bead array, Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP00681-3 targets ASGR1 in immunoassays as a matched antibody pair. Validated in Cytometric bead array, Sandwich ELISA.

Range: 0.625-80 ng/mL (Cytometric Bead Array); 7.8-1000 pg/mL (Sandwich ELISA);

Capture antibody: ASGR1 Recombinant antibody, PBS Only (Capture/Detector) 83691-1-PBS (240672A3). 100 μg. Concentration 1 mgl/ml.

Detection antibody: ASGR1 Recombinant antibody, PBS Only (Detector) 83691-3-PBS (240672C4). 100 μg. Concentration 1 mgl/ml.

Alternative ASGR1 matched antibody pairs: MP00681-1, MP00681-2, MP50623-1

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83691-1-PBS
Tested Reactivity human
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name asialoglycoprotein receptor 1
Synonyms asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1
GenBank accession numberNM_001671.4
Gene symbol ASGR1
UniprotP07306-1
Gene ID (NCBI) 432
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
Catalog Number 83691-3-PBS
Tested Reactivity human, mouse, rat
Immunogen Fusion Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name asialoglycoprotein receptor 1
Synonyms asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1
GenBank accession numberNM_001671.4
Gene symbol ASGR1
UniprotP07306-1
Gene ID (NCBI) 432
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C