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验证数据展示

  • WB analysis using 83691-3-RR (same clone as 83691-3-PBS)

    WB analysis using 83691-3-RR (same clone as 83691-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83691-3-RR (ASGR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83691-3-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 83691-3-RR (same clone as 83691-3-PBS)

    IHC staining of mouse liver using 83691-3-RR (same clone as 83691-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83691-3-RR (ASGR1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83691-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ASGR1 antibody included
  • IHC staining of mouse liver using 83691-3-RR (same clone as 83691-3-PBS)

    IHC staining of mouse liver using 83691-3-RR (same clone as 83691-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83691-3-RR (ASGR1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83691-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ASGR1 antibody included
  • IF Staining of rat liver using 83691-3-RR (same clone as 83691-3-PBS)

    IF Staining of rat liver using 83691-3-RR (same clone as 83691-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using ASGR1 antibody (83691-3-RR, Clone: 240672C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83691-3-PBS in a different storage buffer formulation.

  • IF Staining of rat liver using 83691-3-RR (same clone as 83691-3-PBS)

    IF Staining of rat liver using 83691-3-RR (same clone as 83691-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using ASGR1 antibody (83691-3-RR, Clone: 240672C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83691-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00681-3

    Sandwich ELISA standard curve of MP00681-3

    Sandwich ELISA standard curve of MP00681-3, Human ASGR1 Recombinant Matched Antibody Pair - PBS only. 83691-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0946. 83691-3-PBS was HRP conjugated as the detection antibody. Range: 7.8-1000 pg/mL.

  • Sample test of MP00681-3

    Sample test of MP00681-3

    The mean human ASGR1 concentration was determined to be 175.6 ng/mL in HepG2 cell extract based on a 3.7 mg/mL extract load.

  • Sample test of MP00681-3

    Sample test of MP00681-3

    Serum of sixteen individual healthy human donors was measured. The ASGR1 concentration of detected samples was determined to be 1,563.4 pg/mL with a range of 450.5-3,950.7 pg/mL.

  • Cytometric bead array standard curve of MP00681-1

    Cytometric bead array standard curve of MP00681-1

    Cytometric bead array standard curve of MP00681-1, ASGR1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83691-2-PBS. Detection antibody: 83691-3-PBS. Standard: Eg0946. Range: 0.625-80 ng/mL.

  • Cytometric bead array standard curve of MP00681-3

    Cytometric bead array standard curve of MP00681-3

    Cytometric bead array standard curve of MP00681-3, ASGR1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83691-1-PBS. Detection antibody: 83691-3-PBS. Standard: Eg0946. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83691-3-RR

    Affinity and Kinetic Characterization of 83691-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83691-3-RR against Human ASGR1 were performed. The affinity constant is 1.47 nM.

ASGR1 Recombinant antibody, PBS Only (Detector)

ASGR1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83691-3-PBS

产品说明书

CloneNo. 240672C4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ASGR1 Ready-To-Use IHC Kit. ASGR1 primary antibody included.

产品信息

83691-3-PBS targets ASGR1 as part of a matched antibody pair:

MP00681-1: 83691-2-PBS capture and 83691-3-PBS detection (validated in Cytometric bead array)

MP00681-3: 83691-1-PBS capture and 83691-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 asialoglycoprotein receptor 1
别名 asialoglycoprotein receptor 1, HL-1, Asgpr1, ASGP-R 1, ASGPR 1
计算分子量33 kDa
观测分子量 42-46 kDa
GenBank蛋白编号NM_001671.4
基因名称 ASGR1
Gene ID (NCBI) 432
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP07306-1
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Asialoglycoprotein receptor (ASGPR), also known as the hepatic galactose/N-acetylglucosamine (GlcNAc) receptor or Ashwell receptor, is a C-type lectin expressed exclusively in hepatic parenchymal cells. ASGPR consists of two subunits, a major subunit (ASGR1, HL-1) and a minor subunit (ASGR2, HL-2), and specifically recognizes terminal β-linked galactose or GlcNAc on circulating glycoproteins or cells. This receptor plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins that contain terminal galactose or GlcNAc residues. ASGPR may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.