HRP-conjugated 6*His, His-Tag Monoclonal antibody
6*His, His-Tag Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG1
Reactivity
recombinant protein and More (2)
Applications
WB and More (2)
Conjugate
HRP Fluorescent Dye
验证数据展示
经过测试的应用
| Positive WB detected in | Recombinant protein protein |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Sample-dependent, check data in validation data gallery | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 32 publications below |
| ELISA | See 1 publications below |
产品信息
HRP-66005 targets 6*His, His-Tag in WB, ELISA, Blocking assay applications and shows reactivity with recombinant protein samples.
| Tested Applications | WB |
| Cited Applications | Blocking assay, ELISA, WB |
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human, E. coli |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | 6xHis Peptide |
| Full Name | 6*His, His-Tag |
| Calculated molecular weight | 1 kDa |
| Gene symbol | |
| Gene ID (NCBI) | |
| Conjugate | HRP Fluorescent Dye |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His-tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. This His-tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells. This antibody is conjugated with HRP.
Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HRP 6*His, His-Tag antibody HRP-66005 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Differ STING signaling remodels the tumor microenvironment by antagonizing myeloid-derived suppressor cell expansion. | ||
J Biol Chem The retroviral accessory proteins S2, Nef, and glycoMA use similar mechanisms for antagonizing the host restriction factor SERINC5. | ||
Cell Mol Life Sci Characterization of PPIB interaction in the P3H1 ternary complex and implications for its pathological mutations. | ||
J Biol Chem Characterization of two engineered dimeric Zika virus envelope proteins as immunogen for neutralizing antibody selection and vaccine design. | ||
Anal Chem Receptor-Mediated Field Effect Transistor Biosensor for Real-Time Monitoring of Glutamate Release from Primary Hippocampal Neurons. |
