HRP-conjugated 6*His, His-Tag Monoclonal antibody

6*His, His-Tag Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein and More (2)

Applications

WB and More (2)

Conjugate

HRP Fluorescent Dye

Cat No : HRP-66005

Print datasheet

Synonyms

6*His, 6*His, His Tag, 6histag, 6XHis, HHHHHH, His tag, HISTIDINE TAGGED, HRP 6XHis, HRP HHHHHH, HRP His tag



经过测试的应用

Positive WB detected inRecombinant protein protein

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

产品信息

HRP-66005 targets 6*His, His-Tag in WB, ELISA, Blocking assay applications and shows reactivity with recombinant protein samples.

Tested Applications WB
Cited Applications Blocking assay, ELISA, WB
Tested Reactivity recombinant protein
Cited Reactivityhuman, E. coli
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen 6xHis Peptide
Full Name 6*His, His-Tag
Calculated molecular weight 1 kDa
Gene symbol
Gene ID (NCBI)
Conjugate HRP Fluorescent Dye
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His-tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. This His-tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells. This antibody is conjugated with HRP.

Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.

实验方案

Product Specific Protocols
WB protocol for HRP 6*His, His-Tag antibody HRP-66005Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

PLoS One

PMID: 30677074

Authors - Elizabeth C Upton

Cell Death Differ

STING signaling remodels the tumor microenvironment by antagonizing myeloid-derived suppressor cell expansion.

Authors - Chuan-Xia Zhang

J Biol Chem

The retroviral accessory proteins S2, Nef, and glycoMA use similar mechanisms for antagonizing the host restriction factor SERINC5.

Authors - Iqbal Ahmad

Cell Mol Life Sci

Characterization of PPIB interaction in the P3H1 ternary complex and implications for its pathological mutations.

Authors - Jiawei Wu

J Biol Chem

Characterization of two engineered dimeric Zika virus envelope proteins as immunogen for neutralizing antibody selection and vaccine design.

Authors - Chunpeng Yang

Anal Chem

Receptor-Mediated Field Effect Transistor Biosensor for Real-Time Monitoring of Glutamate Release from Primary Hippocampal Neurons.

Authors - Yu-Tao Li