|Positive WB detected in||Recombinant protein protein|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Sample-dependent, check data in validation data gallery|
HRP-66005 targets 6*His, His-Tag in WB, ELISA, Blocking assay applications and shows reactivity with recombinant protein samples.
|Cited Applications||Blocking assay, ELISA, WB|
|Tested Reactivity||recombinant protein|
|Cited Reactivity||human, E. coli|
|Host / Isotype||Mouse / IgG1|
|Full Name||6*His, His-Tag|
|Calculated molecular weight||1 kDa|
|Gene ID (NCBI)|
|Conjugate||HRP Fluorescent Dye|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.|
|Storage Conditions||Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. His-tag is often used for affinity purification and binding assays. Expressed His-tagged proteins can be purified and detected easily because the string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper, under specific buffer conditions. This His-tag antibody is a useful tool for monitoring of the His-tagged proteins, and recognizes His-tags placed at N-terminal, C-terminal, and internal regions of fusion proteins expressed in bacteria, insect, and mammalian cells. This antibody is conjugated with HRP.
Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.
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