验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, human brain tissue |
| Positive IP detected in | human plasma tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:32000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
66126-1-Ig targets Alpha-2-Macroglobulin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | Alpha-2-Macroglobulin fusion protein Ag19245 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | alpha-2-macroglobulin |
| 别名 | A2M, Alpha 2 M, alpha 2 macroglobulin, Alpha-2-Macroglobulin, CPAMD5, FWP007, S863 7, α-2M |
| 计算分子量 | 1474 aa, 163 kDa |
| 观测分子量 | 185 kDa |
| GenBank蛋白编号 | BC040071 |
| 基因名称 | Alpha 2 Macroglobulin |
| Gene ID (NCBI) | 2 |
| RRID | AB_2881525 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P01023 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
alpha-2-macroglobulin, also known as α2-macroglobulin (α2M and A2M), is a protein abundant in the plasma of vertebrates and several invertebrates. A2M is an evolutionarily conserved arm of the innate immune system. It also mediates the proliferation of T cells and macrophages. A2M acts as a nonspecific protease inhibitor involved in the host defense mechanism that inactivates both endogenous and exogenous proteases, including trypsin, thrombin and collagenase. Even though A2M is produced predominantly by the liver, it may also be expressed in the reproductive tract, heart, and brain, and may have important roles in many physiological processes and medical illnesses including Alzheimer's disease.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Alpha-2-Macroglobulin antibody 66126-1-Ig | Download protocol |
| IHC protocol for Alpha-2-Macroglobulin antibody 66126-1-Ig | Download protocol |
| IF protocol for Alpha-2-Macroglobulin antibody 66126-1-Ig | Download protocol |
| IP protocol for Alpha-2-Macroglobulin antibody 66126-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Am J Cancer Res Prognostic significance of alpha-2-macrglobulin and low-density lipoprotein receptor-related protein-1 in various cancers | ||
Front Physiol Proteomic analyses of urinary exosomes identify novel potential biomarkers for early diagnosis of sickle cell nephropathy, a sex-based study |
