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验证数据展示

  • IF Staining of HepG2 using CL488-66126

    IF Staining of HepG2 using CL488-66126

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL488-66126 (Alpha-2-macroglobulin antibody) at dilution of 1:100.

  • IF Staining of HepG2 using CL488-66126

    IF Staining of HepG2 using CL488-66126

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 Alpha-2-Macroglobulin antibody (CL488-66126, Clone: 4B11F7 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated Alpha-2-Macroglobulin Monoclonal antibody

Alpha-2-Macroglobulin Monoclonal Antibody for IF/ICC
Cat No. CL488-66126

产品说明书

CloneNo. 4B11F7

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IF/ICC

别名

A2M, Alpha 2 M, alpha 2 macroglobulin, Alpha-2-Macroglobulin, CPAMD5, DKFZp779B086, FWP007, S863 7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy A2M Ready-To-Use IHC Kit. A2M primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66126-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66126 targets Alpha-2-Macroglobulin in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 Alpha-2-Macroglobulin fusion protein Ag19245 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 alpha-2-macroglobulin
别名 A2M, Alpha 2 M, alpha 2 macroglobulin, Alpha-2-Macroglobulin, CPAMD5, DKFZp779B086, FWP007, S863 7
计算分子量 1474 aa, 163 kDa
GenBank蛋白编号BC040071
基因名称 Alpha 2 Macroglobulin
Gene ID (NCBI) 2
RRIDAB_2883267
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP01023
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

alpha-2-macroglobulin, also known as α2-macroglobulin (α2M and A2M), is a protein abundant in the plasma of vertebrates and several invertebrates. A2M is an evolutionarily conserved arm of the innate immune system. It also mediates the proliferation of T cells and macrophages. A2M acts as a nonspecific protease inhibitor involved in the host defense mechanism that inactivates both endogenous and exogenous proteases, including trypsin, thrombin and collagenase. Even though A2M is produced predominantly by the liver, it may also be expressed in the reproductive tract, heart, and brain, and may have important roles in many physiological processes and medical illnesses including Alzheimer's disease.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Alpha-2-Macroglobulin antibody CL488-66126Download protocol
Standard Protocols
Click here to view our Standard Protocols