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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis of HepG2 using 66126-1-Ig (same clone as 66126-1-PBS)

    WB analysis of HepG2 using 66126-1-Ig (same clone as 66126-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66126-1-Ig (Alpha-2-macroglobulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66126-1-PBS in a different storage buffer formulation.

  • IP experiment of human plasma using 66126-1-Ig (same clone as 66126-1-PBS)

    IP experiment of human plasma using 66126-1-Ig (same clone as 66126-1-PBS)

    IP result of anti-Alpha-2-Macroglobulin (IP:66126-1-Ig, 5ug; Detection:66126-1-Ig 1:500) with human plasma lysate 4000ug. This data was developed using the same antibody clone with 66126-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66126-1-Ig (same clone as 66126-1-PBS)

    IF Staining of HepG2 using 66126-1-Ig (same clone as 66126-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Alpha-2-Macroglobulin antibody (66126-1-Ig, Clone: 4B11F7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66126-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66126-1-Ig (same clone as 66126-1-PBS)

    IHC staining of human liver using 66126-1-Ig (same clone as 66126-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66126-1-Ig (Alpha-2-Macroglobulin antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66126-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with A2M antibody included
  • IHC staining of human liver using 66126-1-Ig (same clone as 66126-1-PBS)

    IHC staining of human liver using 66126-1-Ig (same clone as 66126-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66126-1-Ig (Alpha-2-Macroglobulin antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66126-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with A2M antibody included

Alpha-2-Macroglobulin Monoclonal antibody, PBS Only

Alpha-2-Macroglobulin Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66126-1-PBS

产品说明书

CloneNo. 4B11F7

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

A2M, Alpha 2 M, alpha 2 macroglobulin, Alpha-2-Macroglobulin, CPAMD5, FWP007, S863 7, α-2M

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy A2M Ready-To-Use IHC Kit. A2M primary antibody included.

产品信息

66126-1-PBS targets Alpha-2-Macroglobulin in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 Alpha-2-Macroglobulin fusion protein Ag19245 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 alpha-2-macroglobulin
别名 A2M, Alpha 2 M, alpha 2 macroglobulin, Alpha-2-Macroglobulin, CPAMD5, FWP007, S863 7, α-2M
计算分子量 1474 aa, 163 kDa
观测分子量 185 kDa
GenBank蛋白编号BC040071
基因名称 Alpha 2 Macroglobulin
Gene ID (NCBI) 2
RRIDAB_2881525
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP01023
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

alpha-2-macroglobulin, also known as α2-macroglobulin (α2M and A2M), is a protein abundant in the plasma of vertebrates and several invertebrates. A2M is an evolutionarily conserved arm of the innate immune system. It also mediates the proliferation of T cells and macrophages. A2M acts as a nonspecific protease inhibitor involved in the host defense mechanism that inactivates both endogenous and exogenous proteases, including trypsin, thrombin and collagenase. Even though A2M is produced predominantly by the liver, it may also be expressed in the reproductive tract, heart, and brain, and may have important roles in many physiological processes and medical illnesses including Alzheimer's disease.