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  • Featured Product
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ABCD1 Monoclonal antibody

ABCD1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1H8E5

Cat no : 60153-1-Ig

Print datasheet

Synonyms

ABC42, ABCD1, Adrenoleukodystrophy protein, ALD, ALDP, AMN

验证数据展示

  • WB analysis of HeLa using 60153-1-Ig

    WB analysis of HeLa using 60153-1-Ig

    WB result of ABCD1 antibody (60153-1-Ig, 1:1000) with si-Control and si-ABCD1 transfected HeLa cells.

  • IF Staining of HeLa using 60153-1-Ig

    IF Staining of HeLa using 60153-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ABCD1 antibody (60153-1-Ig, Clone: 1H8E5 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).DAPI (blue).

经过测试的应用

Positive WB detected inHeLa cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ABCD1 Ready-To-Use IHC Kit. ABCD1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60153-1-Ig targets ABCD1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen ABCD1 fusion protein Ag12757 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ATP-binding cassette, sub-family D (ALD), member 1
Synonyms ABC42, ABCD1, Adrenoleukodystrophy protein, ALD, ALDP, AMN
Calculated Molecular Weight 745 aa, 83 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC025358
Gene Symbol ABCD1
Gene ID (NCBI) 215
RRIDAB_10640436
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP33897
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ABCD1 (also known as ALDP) is a member of the ATP-binding cassette (ABC) transporter superfamily which functions as transporter for a wide variety of substrates. It localizes to the peroxisomal membrane. The exact function is not clear so far. Various mutations of ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), an inherited neurodegenerative disease affecting the nervous system white matter and adrenal cortex.

实验方案

Product Specific Protocols
WB protocol for ABCD1 antibody 60153-1-IgDownload protocol
IF protocol for ABCD1 antibody 60153-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols