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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated ABCD1 Monoclonal antibody

ABCD1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1H8E5

Cat No : CL594-60153

Print datasheet

Synonyms

ABC42, ABCD1, Adrenoleukodystrophy protein, ALD, ALDP, AMN

验证数据展示

  • IF Staining of HeLa using CL594-60153

    IF Staining of HeLa using CL594-60153

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594-conjugated ABCD1 antibody (CL594-60153, Clone: 1H8E5 ) at dilution of 1:100. Green: Stain with CoraLite®488-Phalloidin (CatNo. PF00001).

经过测试的应用

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 60153-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-60153 targets ABCD1 in IF applications and shows reactivity with human samples.

Tested Applications IF/ICC
Tested Reactivity human
Immunogen ABCD1 fusion protein Ag12757 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ATP-binding cassette, sub-family D (ALD), member 1
Synonyms ABC42, ABCD1, Adrenoleukodystrophy protein, ALD, ALDP, AMN
Calculated Molecular Weight 745 aa, 83 kDa
Observed Molecular Weight 75 kDa
GenBank Accession NumberBC025358
Gene Symbol ABCD1
Gene ID (NCBI) 215
RRIDAB_2919897
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP33897
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

ABCD1 (also known as ALDP) is a member of the ATP-binding cassette (ABC) transporter superfamily which functions as transporter for a wide variety of substrates. It localizes to the peroxisomal membrane. The exact function is not clear so far. Various mutations of ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), an inherited neurodegenerative disease affecting the nervous system white matter and adrenal cortex.

实验方案

Product Specific Protocols
IF protocol for CL594 ABCD1 antibody CL594-60153Download protocol
Standard Protocols
Click here to view our Standard Protocols