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验证数据展示

  • IF Staining of U-251 using CL488-12319

    IF Staining of U-251 using CL488-12319

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using CoraLite® Plus 488 ACAA1 antibody (CL488-12319) at dilution of 1:200.

  • FC experiment of U-251 using CL488-12319

    FC experiment of U-251 using CL488-12319

    1x10^6 U-251 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated ACAA1 Polyclonal antibody (CL488-12319)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated ACAA1 Polyclonal antibody

ACAA1 Polyclonal Antibody for IF/ICC
Cat No. CL488-12319

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

Acetyl-CoA C-myristoyltransferase, Acetyl-CoA acyltransferase, Acetyl CoA acyltransferase, ACAA, 3-ketoacyl-CoA thiolase, peroxisomal

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inU-251 cells
Positive FC (Intra) detected inU-251 cells
Planning an IHC experiment? We recommend our IHCeasy ACAA1 Ready-To-Use IHC Kit. ACAA1 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12319 targets ACAA1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 ACAA1 fusion protein Ag2972 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 acetyl-Coenzyme A acyltransferase 1
别名 Acetyl-CoA C-myristoyltransferase, Acetyl-CoA acyltransferase, Acetyl CoA acyltransferase, ACAA, 3-ketoacyl-CoA thiolase, peroxisomal
计算分子量 424 aa, 44 kDa
观测分子量 41 kDa
GenBank蛋白编号BC011977
基因名称 ACAA1
Gene ID (NCBI) 30
RRIDAB_3672536
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP09110
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACAA1 (acetyl-CoA acyltransferase 1) is an enzyme involved in lipid β-oxidation and provides substrates to the tricarboxylic acid (TCA) cycle, a critical step in cellular metabolism (PMID:33194642). ACAA1 is also a biomarker in type 2 diabetes (T2D), predicting the pre-diabetic metabolic signature in mouse models (PMID:25784953). Moreover, ACAA1 is highly expressed in TNBC, serving as a potential therapeutic target in ACAA1-high tumors and a predictive biomarker of resistance to CDK4/6 inhibitors for RB1-proficient patients (PMID:37129951).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ACAA1 antibody CL488-12319Download protocol
FC protocol for CL Plus 488 ACAA1 antibody CL488-12319Download protocol
Standard Protocols
Click here to view our Standard Protocols