• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CoraLite® Plus 488-conjugated ACAA1 Polyclonal antibody

ACAA1 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-12319

Print datasheet

Synonyms

ACAA, ACAA1, Acetyl CoA acyltransferase, Beta ketothiolase, PTHIO, THIO

验证数据展示

  • IF Staining of U-251 using CL488-12319

    IF Staining of U-251 using CL488-12319

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using CoraLite® Plus 488 ACAA1 antibody (CL488-12319) at dilution of 1:200.

经过测试的应用

Positive IF detected inU-251 cells
Planning an IHC experiment? We recommend our IHCeasy ACAA1 Ready-To-Use IHC Kit. ACAA1 primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12319 targets ACAA1 in IF applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen ACAA1 fusion protein Ag2972 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name acetyl-Coenzyme A acyltransferase 1
Synonyms ACAA, ACAA1, Acetyl CoA acyltransferase, Beta ketothiolase, PTHIO, THIO
Calculated Molecular Weight 424 aa, 44 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberBC011977
Gene Symbol ACAA1
Gene ID (NCBI) 30
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09110
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACAA1 (acetyl-CoA acyltransferase 1) is an enzyme involved in lipid β-oxidation and provides substrates to the tricarboxylic acid (TCA) cycle, a critical step in cellular metabolism (PMID:33194642). ACAA1 is also a biomarker in type 2 diabetes (T2D), predicting the pre-diabetic metabolic signature in mouse models (PMID:25784953). Moreover, ACAA1 is highly expressed in TNBC, serving as a potential therapeutic target in ACAA1-high tumors and a predictive biomarker of resistance to CDK4/6 inhibitors for RB1-proficient patients (PMID:37129951).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ACAA1 antibody CL488-12319Download protocol
Standard Protocols
Click here to view our Standard Protocols