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CoraLite® Plus 750-conjugated ACAP1 Recombinant antibody

ACAP1 Recombinant Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230370E1

Cat No : CL750-82967

Print datasheet

Synonyms

验证数据展示

  • WB analysis using CL750-82967

    WB analysis using CL750-82967

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-82967 (ACAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 82967-1-PBS

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-82967 targets ACAP1 in WB applications and shows reactivity with human samples.

Tested Applications WB Application Description
Tested Reactivity human
Immunogen ACAP1 fusion protein Ag34309 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name ArfGAP with coiled-coil, ankyrin repeat and PH domains 1
Synonyms
Calculated Molecular Weight 740 aa, 82 kDa
Observed Molecular Weight 82-85 kDa
GenBank Accession NumberBC018543
Gene Symbol ACAP1
Gene ID (NCBI) 9744
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15027
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACAP1, also named as Centaurin-beta-1, is a 740 amino acid protein, which is expressed Highly in lung and spleen. ACAP1 as a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) is required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface and required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 ACAP1 antibody CL750-82967Download protocol
Standard Protocols
Click here to view our Standard Protocols