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CoraLite® Plus 750-conjugated ACAP1 Recombinant antibody
ACAP1 Uni-rAbTM Recombinant Antibody for WB
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
230370E1
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells |
For other applications, we recommend the unconjugated version of this antibody, 82967-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-82967 targets ACAP1 in WB applications and shows reactivity with human samples.
| Tested Applications | WB Application Description |
| Tested Reactivity | human |
| Immunogen | ACAP1 fusion protein Ag34309 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | ArfGAP with coiled-coil, ankyrin repeat and PH domains 1 |
| Synonyms | |
| Calculated Molecular Weight | 740 aa, 82 kDa |
| Observed Molecular Weight | 82-85 kDa |
| GenBank Accession Number | BC018543 |
| Gene Symbol | ACAP1 |
| Gene ID (NCBI) | 9744 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q15027 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ACAP1, also named as Centaurin-beta-1, is a 740 amino acid protein, which is expressed Highly in lung and spleen. ACAP1 as a GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) is required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface and required for regulated export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 ACAP1 antibody CL750-82967 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |