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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 555-conjugated ACAT2 Polyclonal antibody

ACAT2 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 555 Fluorescent Dye

Cat no : CL555-14755

Print datasheet

Synonyms

EC:2.3.1.9, Cytosolic acetoacetyl-CoA thiolase, ACTL, Acetyl-CoA transferase-like protein, Acetyl-CoA acetyltransferase, cytosolic

验证数据展示

  • FC experiment of HepG2 using CL555-14755

    FC experiment of HepG2 using CL555-14755

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 555 Anti-Human ACAT2 (CL555-14755) (red), or 0.2 ug CoraLite® Plus 555 Rabbit IgG Control Antibody(CL555-30000)(Blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 14755-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-14755 targets ACAT2 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen ACAT2 fusion protein Ag6503 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name acetyl-Coenzyme A acetyltransferase 2
Synonyms EC:2.3.1.9, Cytosolic acetoacetyl-CoA thiolase, ACTL, Acetyl-CoA transferase-like protein, Acetyl-CoA acetyltransferase, cytosolic
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 40-42 kDa
GenBank Accession NumberBC000408
Gene Symbol ACAT2
Gene ID (NCBI) 39
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths554 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BWD1
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Acyl-CoA: cholesterolacyltransferase (ACAT) is an in tracellular membrane-bound enzyme that uses cholesterol and long chain fatty acyl-coenzyme A (CoA) as substrates to produce cholesteryl esters. Two isoforms of the enzyme ACAT, i.e., ACAT1 and ACAT2, are known to exist. ACAT2, which is expressed in liver and intestine cells, has been found to play an important role in intracellular cholesterol homeostasis and univariate survival analyses have shown that ACAT2 expression is associated with a poor clear cell renal cell carcinoma associated survival.

实验方案

Product Specific Protocols
FC protocol for CL Plus 555 ACAT2 antibody CL555-14755Download protocol
Standard Protocols
Click here to view our Standard Protocols