验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 14755-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-14755 targets ACAT2 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | ACAT2 fusion protein Ag6503 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | acetyl-Coenzyme A acetyltransferase 2 |
| 别名 | EC:2.3.1.9, Cytosolic acetoacetyl-CoA thiolase, ACTL, Acetyl-CoA transferase-like protein, Acetyl-CoA acetyltransferase, cytosolic |
| 计算分子量 | 41 kDa |
| 观测分子量 | 40-42 kDa |
| GenBank蛋白编号 | BC000408 |
| 基因名称 | ACAT2 |
| Gene ID (NCBI) | 39 |
| RRID | AB_2934902 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BWD1 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Acyl-CoA: cholesterolacyltransferase (ACAT) is an in tracellular membrane-bound enzyme that uses cholesterol and long chain fatty acyl-coenzyme A (CoA) as substrates to produce cholesteryl esters. Two isoforms of the enzyme ACAT, i.e., ACAT1 and ACAT2, are known to exist. ACAT2, which is expressed in liver and intestine cells, has been found to play an important role in intracellular cholesterol homeostasis and univariate survival analyses have shown that ACAT2 expression is associated with a poor clear cell renal cell carcinoma associated survival.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 ACAT2 antibody CL647-14755 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |