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验证数据展示

  • WB analysis of K-562 using 60176-1-Ig (same clone as 60176-1-PBS)

    WB analysis of K-562 using 60176-1-Ig (same clone as 60176-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 60176-1-Ig (ACPP Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

  • IF Staining of human prostate cancer using 60176-1-Ig (same clone as 60176-1-PBS)

    IF Staining of human prostate cancer using 60176-1-Ig (same clone as 60176-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using ACPP antibody (60176-1-Ig, Clone: 1G2C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

  • IF Staining of human prostate cancer using 60176-1-Ig (same clone as 60176-1-PBS)

    IF Staining of human prostate cancer using 60176-1-Ig (same clone as 60176-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using ACPP antibody (60176-1-Ig, Clone: 1G2C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate hyperplasia using 60176-1-Ig (same clone as 60176-1-PBS)

    IHC staining of human prostate hyperplasia using 60176-1-Ig (same clone as 60176-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 60176-1-Ig (ACPP antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PAP/ACPP antibody included
  • IHC staining of human prostate hyperplasia using 60176-1-Ig (same clone as 60176-1-PBS)

    IHC staining of human prostate hyperplasia using 60176-1-Ig (same clone as 60176-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 60176-1-Ig (ACPP antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PAP/ACPP antibody included

ACPP Monoclonal antibody, PBS Only

ACPP Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 60176-1-PBS

产品说明书

CloneNo. 1G2C9

宿主/亚型

Mouse / IgG2a

种属反应性

human, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

acid phosphatase, prostate, ACP 3, ACP3, ACPP, PAP, Prostatic acid phosphatase

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PAP/ACPP Ready-To-Use IHC Kit. PAP/ACPP primary antibody included.

产品信息

60176-1-PBS targets ACPP in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 ACPP fusion protein Ag8578 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 acid phosphatase, prostate
别名 acid phosphatase, prostate, ACP 3, ACP3, ACPP, PAP, Prostatic acid phosphatase
计算分子量 418 aa, 48 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC007460
基因名称 ACPP
Gene ID (NCBI) 55
RRIDAB_10733336
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP15309
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase,thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042). This protein has three glycosylation sites(PMID:10639192;12525165).