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ACPP Monoclonal antibody, PBS Only

ACPP Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G2C9

Cat No : 60176-1-PBS

Print datasheet

Synonyms

acid phosphatase, prostate, ACP 3, ACP3, ACPP, PAP, Prostatic acid phosphatase

验证数据展示

  • WB analysis of K-562 using 60176-1-Ig (same clone as 60176-1-PBS)

    WB analysis of K-562 using 60176-1-Ig (same clone as 60176-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 60176-1-Ig (ACPP Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

  • IF Staining of human prostate cancer using 60176-1-Ig (same clone as 60176-1-PBS)

    IF Staining of human prostate cancer using 60176-1-Ig (same clone as 60176-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using ACPP antibody (60176-1-Ig, Clone: 1G2C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

  • IF Staining of human prostate cancer using 60176-1-Ig (same clone as 60176-1-PBS)

    IF Staining of human prostate cancer using 60176-1-Ig (same clone as 60176-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using ACPP antibody (60176-1-Ig, Clone: 1G2C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate hyperplasia using 60176-1-Ig (same clone as 60176-1-PBS)

    IHC staining of human prostate hyperplasia using 60176-1-Ig (same clone as 60176-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 60176-1-Ig (ACPP antibody) at dilution of 1:1000 (under 10x lens). This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PAP/ACPP antibody included
  • IHC staining of human prostate hyperplasia using 60176-1-Ig (same clone as 60176-1-PBS)

    IHC staining of human prostate hyperplasia using 60176-1-Ig (same clone as 60176-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 60176-1-Ig (ACPP antibody) at dilution of 1:1000 (under 40x lens). This data was developed using the same antibody clone with 60176-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PAP/ACPP antibody included
Planning an IHC experiment? We recommend our IHCeasy PAP/ACPP Ready-To-Use IHC Kit. PAP/ACPP primary antibody included.

产品信息

60176-1-PBS targets ACPP in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IF, IHC, Indirect ELISA Application Description
Tested Reactivity human, rat
Immunogen ACPP fusion protein Ag8578 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name acid phosphatase, prostate
Synonyms acid phosphatase, prostate, ACP 3, ACP3, ACPP, PAP, Prostatic acid phosphatase
Calculated Molecular Weight 418 aa, 48 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC007460
Gene Symbol ACPP
Gene ID (NCBI) 55
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP15309
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

ACPP is also named as PAP(prostatic acid phosphatase),5'-nucleotidase,thiamine monophosphatas and Belongs to the histidine acid phosphatase family.This enzyme is a homodimeric glycoprotein with a subunit molecular mass of 50 kDa and is an androgen-stimulated secretory product of the human prostate gland(PMID:8132635).It is also a non-specific phosphatase and has been implicated in the progression of prostate cancer, which inactivates LPA in human seminal plasma(PMID:15280042). This protein has three glycosylation sites(PMID:10639192;12525165).