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ACSS2 Recombinant antibody, PBS Only

ACSS2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242253C6

Cat no : 84774-7-PBS

Print datasheet

Synonyms

Acetyl-CoA synthetase 1, Acetyl-CoA synthetase, Acetate--CoA ligase, AceCS1, ACAS2

验证数据展示

  • WB analysis using 84774-7-RR (same clone as 84774-7-PBS)

    WB analysis using 84774-7-RR (same clone as 84774-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84774-7-RR (ACSS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84774-7-PBS in a different storage buffer formulation.

  • FC experiment of Caco-2 using 84774-7-RR (same clone as 84774-7-PBS)

    FC experiment of Caco-2 using 84774-7-RR (same clone as 84774-7-PBS)

    1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug ACSS2 Recombinant antibody (84774-7-RR, Clone:242253C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84774-7-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 84774-7-RR (same clone as 84774-7-PBS)

    FC experiment of HepG2 using 84774-7-RR (same clone as 84774-7-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ACSS2 Recombinant antibody (84774-7-RR, Clone:242253C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84774-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84774-7-RR

    Affinity and Kinetic Characterization of 84774-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84774-7-RR against Human ACSS2 were performed. The affinity constant is 91.2 nM.

产品信息

84774-7-PBS targets ACSS2 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ACSS2 fusion protein Ag9121 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name acyl-CoA synthetase short-chain family member 2
Synonyms Acetyl-CoA synthetase 1, Acetyl-CoA synthetase, Acetate--CoA ligase, AceCS1, ACAS2
Calculated Molecular Weight 701 aa, 79 kDa
Observed Molecular Weight 79 kDa
GenBank Accession NumberBC010141
Gene Symbol ACSS2
Gene ID (NCBI) 55902
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9NR19
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Acetyl-CoA Synthetase 2 (ACSS2) is also named as acyl-CoA synthetase short-chain family member 2. Acetyl-CoA Synthetase 2 (ACSS2) is responsible for lipogenesis by synthesizing acetyl-CoA from acetate for lipogenesis and promotes acetylation in nuclear (PMID: 29444517). It is also required for cell migration and invasion in renal cell carcinoma (RCC) through the promotion of autophagy (PMID: 29281714).