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验证数据展示

  • WB analysis using 84774-7-RR

    WB analysis using 84774-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84774-7-RR (ACSS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of Caco-2 using 84774-7-RR

    FC experiment of Caco-2 using 84774-7-RR

    1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug ACSS2 Recombinant antibody (84774-7-RR, Clone:242253C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HepG2 using 84774-7-RR

    FC experiment of HepG2 using 84774-7-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ACSS2 Recombinant antibody (84774-7-RR, Clone:242253C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84774-7-RR

    Affinity and Kinetic Characterization of 84774-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84774-7-RR against Human ACSS2 were performed. The affinity constant is 91.2 nM.

ACSS2 Recombinant antibody

ACSS2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84774-7-RR

产品说明书

CloneNo. 242253C6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

Acetyl-CoA synthetase 1, Acetyl-CoA synthetase, Acetate--CoA ligase, AceCS1, ACAS2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, Caco-2 cells, mouse liver tissue, rat liver tissue, mouse brain tissue, rat brain tissue
Positive FC (Intra) detected inHepG2 cells, Caco-2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84774-7-RR targets ACSS2 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ACSS2 fusion protein Ag9121 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 acyl-CoA synthetase short-chain family member 2
别名 Acetyl-CoA synthetase 1, Acetyl-CoA synthetase, Acetate--CoA ligase, AceCS1, ACAS2
计算分子量 701 aa, 79 kDa
观测分子量 79 kDa
GenBank蛋白编号BC010141
基因名称 ACSS2
Gene ID (NCBI) 55902
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9NR19
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Acetyl-CoA Synthetase 2 (ACSS2) is also named as acyl-CoA synthetase short-chain family member 2. Acetyl-CoA Synthetase 2 (ACSS2) is responsible for lipogenesis by synthesizing acetyl-CoA from acetate for lipogenesis and promotes acetylation in nuclear (PMID: 29444517). It is also required for cell migration and invasion in renal cell carcinoma (RCC) through the promotion of autophagy (PMID: 29281714).

实验方案

Product Specific Protocols
WB protocol for ACSS2 antibody 84774-7-RRDownload protocol
FC protocol for ACSS2 antibody 84774-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols