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ACSS2 Recombinant antibody

ACSS2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

242253C6

Cat no : 84774-7-RR

Print datasheet

Synonyms

Acetyl-CoA synthetase 1, Acetyl-CoA synthetase, Acetate--CoA ligase, AceCS1, ACAS2

验证数据展示

  • WB analysis using 84774-7-RR

    WB analysis using 84774-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84774-7-RR (ACSS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of Caco-2 using 84774-7-RR

    FC experiment of Caco-2 using 84774-7-RR

    1x10^6 Caco-2 cells were intracellularly stained with 0.25 ug ACSS2 Recombinant antibody (84774-7-RR, Clone:242253C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of HepG2 using 84774-7-RR

    FC experiment of HepG2 using 84774-7-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug ACSS2 Recombinant antibody (84774-7-RR, Clone:242253C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84774-7-RR

    Affinity and Kinetic Characterization of 84774-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84774-7-RR against Human ACSS2 were performed. The affinity constant is 91.2 nM.

经过测试的应用

Positive WB detected inHepG2 cells, Caco-2 cells, mouse liver tissue, rat liver tissue, mouse brain tissue, rat brain tissue
Positive FC (Intra) detected inHepG2 cells, Caco-2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84774-7-RR targets ACSS2 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ACSS2 fusion protein Ag9121 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name acyl-CoA synthetase short-chain family member 2
Synonyms Acetyl-CoA synthetase 1, Acetyl-CoA synthetase, Acetate--CoA ligase, AceCS1, ACAS2
Calculated Molecular Weight 701 aa, 79 kDa
Observed Molecular Weight 79 kDa
GenBank Accession NumberBC010141
Gene Symbol ACSS2
Gene ID (NCBI) 55902
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9NR19
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Acetyl-CoA Synthetase 2 (ACSS2) is also named as acyl-CoA synthetase short-chain family member 2. Acetyl-CoA Synthetase 2 (ACSS2) is responsible for lipogenesis by synthesizing acetyl-CoA from acetate for lipogenesis and promotes acetylation in nuclear (PMID: 29444517). It is also required for cell migration and invasion in renal cell carcinoma (RCC) through the promotion of autophagy (PMID: 29281714).

实验方案

Product Specific Protocols
WB protocol for ACSS2 antibody 84774-7-RRDownload protocol
FC protocol for ACSS2 antibody 84774-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols