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ADH1B Monoclonal antibody

ADH1B Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66939-1-Ig

产品说明书

CloneNo. 1F1B4

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse, Rat, Pig and More (2)

应用

WB, IHC, IF/ICC, ELISA and More (1)

ADH1B, Alcohol dehydrogenase 1B, DKFZp686C06125

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inL02 cells, HepG2 cells, pig liver tissue, rat liver tissue, SMMC-7721 cells, HuH-7 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ADH1B Ready-To-Use IHC Kit. ADH1B primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66939-1-Ig targets ADH1B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 Human, Mouse, Rat, Pig
文献引用反应性human, mouse
免疫原 ADH1B fusion protein Ag10630 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 alcohol dehydrogenase 1B (class I), beta polypeptide
别名 ADH1B, Alcohol dehydrogenase 1B, DKFZp686C06125
计算分子量 375 aa, 40 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC033009
基因名称 ADH1B
Gene ID (NCBI) 125
RRIDAB_2882263
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP00325
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ADH1B gene encodes the beta subunit of class I alcohol dehydrogenase (ADH), an enzyme that catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde. Class 1 ADH is a homo- or heterodimeric molecule, formed by the association of 3 types of class I ADH subunits, alpha (ADH1A), beta, and gamma(ADH1C). ADH1B is also named as ADH2 and belongs to the zinc-containing alcohol dehydrogenase family. This protein can exsit as a homodimer(PMID:19365573).

实验方案

Product Specific Protocols
WB protocol for ADH1B antibody 66939-1-IgDownload protocol
IHC protocol for ADH1B antibody 66939-1-IgDownload protocol
IF protocol for ADH1B antibody 66939-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC

iScience

A positive feed-forward loop between Fusobacterium nucleatum and ethanol metabolism reprogramming drives laryngeal cancer progression and metastasis.

Authors - Chi-Yao Hsueh
mouseWB

Front Pharmacol

Kinsenoside Alleviates Alcoholic Liver Injury by Reducing Oxidative Stress, Inhibiting Endoplasmic Reticulum Stress, and Regulating AMPK-Dependent Autophagy.

Authors - Limin Gao
humanWB

Front Genet

Analysis of characteristic genes and ceRNA regulation mechanism of endometriosis based on full transcriptional sequencing

Authors - Chengmao Xie
humanIHC

Cell Rep Methods

Modeling alcohol-associated liver disease in humans using adipose stromal or stem cell-derived organoids

Authors - Guoyun Bi
humanIF

Oncoimmunology

HIF2A mediates lineage transition to aggressive phenotype of cancer-associated fibroblasts in lung cancer brain metastasis

Authors - Muyuan You
humanIF

Cancer Commun (Lond)

Nicotinamide N-methyltransferase negatively regulates metastasis-promoting property of cancer-associated fibroblasts in lung adenocarcinoma

Authors - Peiyu Wang
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