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验证数据展示

  • WB analysis using 66939-1-Ig (same clone as 66939-1-PBS)

    WB analysis using 66939-1-Ig (same clone as 66939-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66939-1-Ig (ADH1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66939-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66939-1-Ig (same clone as 66939-1-PBS)

    WB analysis of HepG2 using 66939-1-Ig (same clone as 66939-1-PBS)

    WB result of ADH1B antibody (66939-1-Ig; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ADH1B transfected HepG2 cells. This data was developed using the same antibody clone with 66939-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66939-1-Ig (same clone as 66939-1-PBS)

    IHC staining of human liver cancer using 66939-1-Ig (same clone as 66939-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66939-1-Ig (ADH1B antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66939-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ADH1B antibody included
  • IHC staining of human liver cancer using 66939-1-Ig (same clone as 66939-1-PBS)

    IHC staining of human liver cancer using 66939-1-Ig (same clone as 66939-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66939-1-Ig (ADH1B antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66939-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ADH1B antibody included
  • IF Staining of HepG2 using 66939-1-Ig (same clone as 66939-1-PBS)

    IF Staining of HepG2 using 66939-1-Ig (same clone as 66939-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ADH1B antibody (66939-1-Ig, Clone: 1F1B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66939-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

ADH1B Monoclonal antibody, PBS Only

ADH1B Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66939-1-PBS

产品说明书

CloneNo. 1F1B4

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse, Rat, Pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

ADH1B, Alcohol dehydrogenase 1B, DKFZp686C06125

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ADH1B Ready-To-Use IHC Kit. ADH1B primary antibody included.

产品信息

66939-1-PBS targets ADH1B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat, Pig
免疫原 ADH1B fusion protein Ag10630 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 alcohol dehydrogenase 1B (class I), beta polypeptide
别名 ADH1B, Alcohol dehydrogenase 1B, DKFZp686C06125
计算分子量 375 aa, 40 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC033009
基因名称 ADH1B
Gene ID (NCBI) 125
RRIDAB_2882263
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP00325
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The ADH1B gene encodes the beta subunit of class I alcohol dehydrogenase (ADH), an enzyme that catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde. Class 1 ADH is a homo- or heterodimeric molecule, formed by the association of 3 types of class I ADH subunits, alpha (ADH1A), beta, and gamma(ADH1C). ADH1B is also named as ADH2 and belongs to the zinc-containing alcohol dehydrogenase family. This protein can exsit as a homodimer(PMID:19365573).