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  • Featured Product
  • KD/KO Validated

ADH1B Monoclonal antibody, PBS Only

ADH1B Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F1B4

Cat no : 66939-1-PBS

Print datasheet

Synonyms

ADH1B, Alcohol dehydrogenase 1B, DKFZp686C06125

验证数据展示

  • WB analysis using 66939-1-Ig (same clone as 66939-1-PBS)

    WB analysis using 66939-1-Ig (same clone as 66939-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66939-1-Ig (ADH1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66939-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 66939-1-Ig (same clone as 66939-1-PBS)

    WB analysis of HepG2 using 66939-1-Ig (same clone as 66939-1-PBS)

    WB result of ADH1B antibody (66939-1-Ig; 1:50000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ADH1B transfected HepG2 cells. This data was developed using the same antibody clone with 66939-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66939-1-Ig (same clone as 66939-1-PBS)

    IHC staining of human liver cancer using 66939-1-Ig (same clone as 66939-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66939-1-Ig (ADH1B antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66939-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ADH1B antibody included
  • IHC staining of human liver cancer using 66939-1-Ig (same clone as 66939-1-PBS)

    IHC staining of human liver cancer using 66939-1-Ig (same clone as 66939-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66939-1-Ig (ADH1B antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66939-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ADH1B antibody included
  • IF Staining of HepG2 using 66939-1-Ig (same clone as 66939-1-PBS)

    IF Staining of HepG2 using 66939-1-Ig (same clone as 66939-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ADH1B antibody (66939-1-Ig, Clone: 1F1B4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66939-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ADH1B Ready-To-Use IHC Kit. ADH1B primary antibody included.

产品信息

66939-1-PBS targets ADH1B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen ADH1B fusion protein Ag10630 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name alcohol dehydrogenase 1B (class I), beta polypeptide
Synonyms ADH1B, Alcohol dehydrogenase 1B, DKFZp686C06125
Calculated Molecular Weight 375 aa, 40 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC033009
Gene Symbol ADH1B
Gene ID (NCBI) 125
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00325
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The ADH1B gene encodes the beta subunit of class I alcohol dehydrogenase (ADH), an enzyme that catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde. Class 1 ADH is a homo- or heterodimeric molecule, formed by the association of 3 types of class I ADH subunits, alpha (ADH1A), beta, and gamma(ADH1C). ADH1B is also named as ADH2 and belongs to the zinc-containing alcohol dehydrogenase family. This protein can exsit as a homodimer(PMID:19365573).