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验证数据展示

  • IF Staining of HepG2 using CL488-66939

    IF Staining of HepG2 using CL488-66939

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 ADH1B antibody (CL488-66939, Clone: 1F1B4 ) at dilution of 1:200, CL594-Phalloidin (red). DAPI (blue).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated ADH1B Monoclonal antibody

ADH1B Monoclonal Antibody for IF/ICC
Cat No. CL488-66939

产品说明书

CloneNo. 1F1B4

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse, Rat, Pig

应用

IF/ICC

别名

ADH1B, Alcohol dehydrogenase 1B, DKFZp686C06125

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ADH1B Ready-To-Use IHC Kit. ADH1B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66939-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66939 targets ADH1B in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Pig samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, Mouse, Rat, Pig
免疫原 ADH1B fusion protein Ag10630 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 alcohol dehydrogenase 1B (class I), beta polypeptide
别名 ADH1B, Alcohol dehydrogenase 1B, DKFZp686C06125
计算分子量 375 aa, 40 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC033009
基因名称 ADH1B
Gene ID (NCBI) 125
RRIDAB_2919392
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长488 nm / 515 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP00325
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ADH1B gene encodes the beta subunit of class I alcohol dehydrogenase (ADH), an enzyme that catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde. Class 1 ADH is a homo- or heterodimeric molecule, formed by the association of 3 types of class I ADH subunits, alpha (ADH1A), beta, and gamma(ADH1C). ADH1B is also named as ADH2 and belongs to the zinc-containing alcohol dehydrogenase family. This protein can exsit as a homodimer(PMID:19365573).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ADH1B antibody CL488-66939Download protocol
Standard Protocols
Click here to view our Standard Protocols