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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated ADH1B Monoclonal antibody

ADH1B Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat, Pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F1B4

Cat no : CL488-66939

Print datasheet

Synonyms

ADH1B, Alcohol dehydrogenase 1B, DKFZp686C06125

验证数据展示

  • IF Staining of HepG2 using CL488-66939

    IF Staining of HepG2 using CL488-66939

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 ADH1B antibody (CL488-66939, Clone: 1F1B4 ) at dilution of 1:200, CL594-Phalloidin (red). DAPI (blue).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ADH1B Ready-To-Use IHC Kit. ADH1B primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66939-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66939 targets ADH1B in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen ADH1B fusion protein Ag10630 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name alcohol dehydrogenase 1B (class I), beta polypeptide
Synonyms ADH1B, Alcohol dehydrogenase 1B, DKFZp686C06125
Calculated Molecular Weight 375 aa, 40 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC033009
Gene Symbol ADH1B
Gene ID (NCBI) 125
RRIDAB_2919392
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths488 nm / 515 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP00325
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ADH1B gene encodes the beta subunit of class I alcohol dehydrogenase (ADH), an enzyme that catalyzes the rate-limiting step for ethanol metabolism: the oxidation of alcohol to acetaldehyde. Class 1 ADH is a homo- or heterodimeric molecule, formed by the association of 3 types of class I ADH subunits, alpha (ADH1A), beta, and gamma(ADH1C). ADH1B is also named as ADH2 and belongs to the zinc-containing alcohol dehydrogenase family. This protein can exsit as a homodimer(PMID:19365573).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ADH1B antibody CL488-66939Download protocol
Standard Protocols
Click here to view our Standard Protocols