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验证数据展示

  • FC experiment of HeLa using CL488-10658

    FC experiment of HeLa using CL488-10658

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Ahcyl1 Polyclonal Antibody (CL488-10658)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL488-10658

    IF Staining of HeLa using CL488-10658

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 AHCYL1 antibody (CL488-10658) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated AHCYL1 Polyclonal antibody

AHCYL1 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-10658

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

S-adenosylhomocysteine hydrolase-like protein 1, IRBIT, IP(3)Rs binding protein released with IP(3), DC-expressed AHCY-like molecule, DCAL

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy AHCYL1 Ready-To-Use IHC Kit. AHCYL1 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10658 targets AHCYL1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 AHCYL1 fusion protein Ag1017 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 S-adenosylhomocysteine hydrolase-like 1
别名 S-adenosylhomocysteine hydrolase-like protein 1, IRBIT, IP(3)Rs binding protein released with IP(3), DC-expressed AHCY-like molecule, DCAL
计算分子量 65 kDa
观测分子量 60 kDa
GenBank蛋白编号BC007576
基因名称 AHCYL1
Gene ID (NCBI) 10768
RRIDAB_3672458
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43865
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S-adenosylhomocysteine hydrolase-like protein 1 (AHCYL1), also known as IP3 receptor-binding protein released with IP3 (IRBIT), regulates IP3-induced Ca2+ release into the cytoplasm of cells(PMID:23145124). This protein is also named as DCAL, XPVKONA and belongs to the adenosylhomocysteinase family. AHCYL1 mRNA and protein are most abundant in the glandular epithelium of adenocarcinoma of cancerous, but not normal and AHCYL1 protein is predominantly in the nucleus(PMID:22826361). It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 AHCYL1 antibody CL488-10658Download protocol
FC protocol for CL Plus 488 AHCYL1 antibody CL488-10658Download protocol
Standard Protocols
Click here to view our Standard Protocols