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验证数据展示

  • FC experiment of HeLa using CL488-10658

    FC experiment of HeLa using CL488-10658

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Ahcyl1 Polyclonal Antibody (CL488-10658)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using CL488-10658

    IF Staining of HeLa using CL488-10658

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 AHCYL1 antibody (CL488-10658) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated AHCYL1 Polyclonal antibody

AHCYL1 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-10658

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Synonyms

S-adenosylhomocysteine hydrolase-like protein 1, IRBIT, IP(3)Rs binding protein released with IP(3), DC-expressed AHCY-like molecule, DCAL

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy AHCYL1 Ready-To-Use IHC Kit. AHCYL1 primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10658 targets AHCYL1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen AHCYL1 fusion protein Ag1017 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name S-adenosylhomocysteine hydrolase-like 1
Synonyms S-adenosylhomocysteine hydrolase-like protein 1, IRBIT, IP(3)Rs binding protein released with IP(3), DC-expressed AHCY-like molecule, DCAL
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC007576
Gene Symbol AHCYL1
Gene ID (NCBI) 10768
RRIDAB_3672458
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43865
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

S-adenosylhomocysteine hydrolase-like protein 1 (AHCYL1), also known as IP3 receptor-binding protein released with IP3 (IRBIT), regulates IP3-induced Ca2+ release into the cytoplasm of cells(PMID:23145124). This protein is also named as DCAL, XPVKONA and belongs to the adenosylhomocysteinase family. AHCYL1 mRNA and protein are most abundant in the glandular epithelium of adenocarcinoma of cancerous, but not normal and AHCYL1 protein is predominantly in the nucleus(PMID:22826361). It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 AHCYL1 antibody CL488-10658Download protocol
FC protocol for CL Plus 488 AHCYL1 antibody CL488-10658Download protocol
Standard Protocols
Click here to view our Standard Protocols