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验证数据展示

  • IHC staining of mouse testis using 83369-6-RR (same clone as 83369-6-PBS)

    IHC staining of mouse testis using 83369-6-RR (same clone as 83369-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83369-6-RR (AHR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83369-6-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AHR antibody included
  • IF Staining of HepG2 using 83369-6-RR (same clone as 83369-6-PBS)

    IF Staining of HepG2 using 83369-6-RR (same clone as 83369-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AHR antibody (83369-6-RR, Clone: 240325G9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83369-6-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83369-6-RR (same clone as 83369-6-PBS)

    IF Staining of HeLa using 83369-6-RR (same clone as 83369-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AHR antibody (83369-6-RR, Clone: 240325G9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83369-6-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83369-6-RR (same clone as 83369-6-PBS)

    IF Staining of HeLa using 83369-6-RR (same clone as 83369-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AHR antibody (83369-6-RR, Clone: 240325G9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83369-6-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83369-6-RR (same clone as 83369-6-PBS)

    FC experiment of HeLa using 83369-6-RR (same clone as 83369-6-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug AHR Recombinant antibody (83369-6-RR, Clone:240325G9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83369-6-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 83369-6-RR (same clone as 83369-6-PBS)

    FC experiment of MCF-7 using 83369-6-RR (same clone as 83369-6-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug AHR Recombinant antibody (83369-6-RR, Clone:240325G9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83369-6-PBS in a different storage buffer formulation.

AHR Recombinant antibody, PBS Only

AHR Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA
Cat No. 83369-6-PBS

产品说明书

CloneNo. 240325G9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, FC (Intra), ELISA

别名

Class E basic helix-loop-helix protein 76, bHLHe76, Ah receptor, 240325G9

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy AHR Ready-To-Use IHC Kit. AHR primary antibody included.

产品信息

83369-6-PBS targets AHR in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 AHR fusion protein Ag12193 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 aryl hydrocarbon receptor
别名 Class E basic helix-loop-helix protein 76, bHLHe76, Ah receptor, 240325G9
计算分子量 848 aa, 96 kDa
GenBank蛋白编号BC070080
基因名称 AHR
Gene ID (NCBI) 196
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP35869
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The aryl hydrocarbon receptor (AhR) is a ligand-activated transcription factor that has been largely regarded as a mediator of xenobiotic metabolism (PMID:18483242). It plays a part role in physiologic activities, including attenuation of the acute phase response, cytokine signaling, T helper (TH) 17 immune cell differentiation, modulation of NF-κB activity, and regulation of hormonal signaling (PMID:20423157,18540824). It also mediates transcription factor sequestering away from a gene promoter or tethering of the AhR to a transcription factor on a promoter. AHR calculated molecular masses differ by <10%, compared with the apparent molecular masses predicted from SDS-PAGE for the two receptors (105 and 95 kDa, respectively). (PMID: 8246913)