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验证数据展示

  • IF Staining of NIH/3T3 using CL488-28113

    IF Staining of NIH/3T3 using CL488-28113

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CoraLite® Plus 488 AKT2 antibody (CL488-28113) at dilution of 1:200.

  • FC experiment of NIH/3T3 using CL488-28113

    FC experiment of NIH/3T3 using CL488-28113

    1x10^6 NIH/3T3 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Akt2 Polyclonal Antibody (CL488-28113)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated AKT2 Polyclonal antibody

AKT2 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-28113

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

IF/ICC, FC (Intra)

别名

PKBBETA, PKBB, PKB beta, EC:2.7.11.1, AKT2 protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inNIH/3T3 cells
For other applications, we recommend the unconjugated version of this antibody, 28113-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-28113 targets AKT2 in IF/ICC, FC (Intra) applications and shows reactivity with Human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 Human, mouse
免疫原 AKT2 fusion protein Ag27005 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 v-akt murine thymoma viral oncogene homolog 2
别名 PKBBETA, PKBB, PKB beta, EC:2.7.11.1, AKT2 protein
观测分子量56 kDa
GenBank蛋白编号BC120994
基因名称 AKT2
Gene ID (NCBI) 208
RRIDAB_3672822
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP31751
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis and their activation has been observed in a wide variety of cancers. AKT2 is mainly involved in cancer cell survival, apoptosis inhibition, migration and invasion(PMID:21979951). Defects in AKT2 are a cause of susceptibility to breast cancer (BC). AKT2 promotes metastasis of tumor cells without affecting the latency of tumor development. And defects in AKT2 are a cause of non-insulin-dependent diabetes mellitus (NIDDM) and hypoinsulinemic hypoglycemia with hemihypertrophy (HIHGHH). The full length protein has four glycosylation sites.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 AKT2 antibody CL488-28113Download protocol
FC protocol for CL Plus 488 AKT2 antibody CL488-28113Download protocol
Standard Protocols
Click here to view our Standard Protocols