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验证数据展示

  • IF Staining of NIH/3T3 using CL488-28113

    IF Staining of NIH/3T3 using CL488-28113

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using CoraLite® Plus 488 AKT2 antibody (CL488-28113) at dilution of 1:200.

  • FC experiment of NIH/3T3 using CL488-28113

    FC experiment of NIH/3T3 using CL488-28113

    1x10^6 NIH/3T3 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Akt2 Polyclonal Antibody (CL488-28113)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite® Plus 488-conjugated AKT2 Polyclonal antibody

AKT2 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-28113

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IF/ICC, FC (Intra)

Synonyms

PKBBETA, PKBB, PKB beta, EC:2.7.11.1, AKT2 protein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inNIH/3T3 cells
For other applications, we recommend the unconjugated version of this antibody, 28113-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-28113 targets AKT2 in IF/ICC, FC (Intra) applications and shows reactivity with Human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity Human, mouse
Immunogen AKT2 fusion protein Ag27005 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name v-akt murine thymoma viral oncogene homolog 2
Synonyms PKBBETA, PKBB, PKB beta, EC:2.7.11.1, AKT2 protein
Observed Molecular Weight56 kDa
GenBank Accession NumberBC120994
Gene Symbol AKT2
Gene ID (NCBI) 208
RRIDAB_3672822
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31751
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AKT2 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis and their activation has been observed in a wide variety of cancers. AKT2 is mainly involved in cancer cell survival, apoptosis inhibition, migration and invasion(PMID:21979951). Defects in AKT2 are a cause of susceptibility to breast cancer (BC). AKT2 promotes metastasis of tumor cells without affecting the latency of tumor development. And defects in AKT2 are a cause of non-insulin-dependent diabetes mellitus (NIDDM) and hypoinsulinemic hypoglycemia with hemihypertrophy (HIHGHH). The full length protein has four glycosylation sites.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 AKT2 antibody CL488-28113Download protocol
FC protocol for CL Plus 488 AKT2 antibody CL488-28113Download protocol
Standard Protocols
Click here to view our Standard Protocols