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验证数据展示

  • FC experiment of human PBMCs using CL555-65223

    FC experiment of human PBMCs using CL555-65223

    1X10^6 PHA-treated (day 3) human PBMCs were surface stained with 5 ul CoraLite® Plus 555 Anti-Human ALCAM (CL555-65223, Clone:3A6) (red), or unstained (blue). The black dashed line indicates unactivated PBMCs surface stained with 5 ul CoraLite® Plus 555 Anti-Human ALCAM (CL555-65223, Clone:3A6). Cells were not fixed. Monocytes were gated.

CoraLite® Plus 555 Anti-Human ALCAM (3A6)

ALCAM Monoclonal Antibody for FC
Cat No. CL555-65223
CloneNo. 3A6

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Synonyms

ALCAM, CD166, CD166 antigen, FLJ38514, MEMD

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 555
Size: 

-/ -

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经过测试的应用

Positive FC detected inPHA-treated human PBMCs
For other applications, we recommend the unconjugated version of this antibody, 65223-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-65223 targets ALCAM in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen Cultured human thymic epithelial cells 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name activated leukocyte cell adhesion molecule
Synonyms ALCAM, CD166, CD166 antigen, FLJ38514, MEMD
Calculated Molecular Weight 105 kDa
GenBank Accession NumberBC057809
Gene Symbol ALCAM
Gene ID (NCBI) 214
RRIDAB_3084600
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths554 nm / 570 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDQ13740
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues and cell types, including activated T cells and monocytes, epithelial cells, fibroblasts, neuronal cells, hepatocytes, and bone marrow mesenchymal stem cells (PMID: 7760007; 25221999). Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).

实验方案

Product Specific Protocols
FC protocol for CL Plus 555 ALCAM antibody CL555-65223Download protocol
Standard Protocols
Click here to view our Standard Protocols