CoraLite® Plus 647 Anti-Human ALCAM (3A6)
ALCAM Monoclonal Antibody for FC
Host / Isotype
Mouse / IgG1, kappa
Reactivity
Human
Applications
FC
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
3A6
验证数据展示
经过测试的应用
| Positive FC detected in | PHA treated human PBMCs |
For other applications, we recommend the unconjugated version of this antibody, 65223-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-65223 targets ALCAM in FC applications and shows reactivity with Human samples.
| Tested Applications | FC |
| Tested Reactivity | Human |
| Immunogen | Cultured human thymic epithelial cells 种属同源性预测 |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | activated leukocyte cell adhesion molecule |
| Synonyms | ALCAM, CD166, CD166 antigen, FLJ38514, MEMD |
| Calculated Molecular Weight | 105 kDa |
| GenBank Accession Number | BC057809 |
| Gene Symbol | ALCAM |
| Gene ID (NCBI) | 214 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | Q13740 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues and cell types, including activated T cells and monocytes, epithelial cells, fibroblasts, neuronal cells, hepatocytes, and bone marrow mesenchymal stem cells (PMID: 7760007; 25221999). Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 ALCAM antibody CL647-65223 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
