验证数据展示
经过测试的应用
| Positive WB detected in | L02 cells |
| Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy ALPL Ready-To-Use IHC Kit. ALPL primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82915-2-RR targets ALPL in WB, FC (Intra), ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, FC (Intra), ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | ALPL fusion protein Ag1664 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | alkaline phosphatase, liver/bone/kidney |
| 别名 | Alkaline phosphatase, Alkaline phosphatase, tissue-nonspecific isozyme, Alkaline phosphatase tissue nonspecific isozyme, Alkaline phosphatase liver/bone/kidney isozyme, AKP2 |
| 计算分子量 | 57 kDa |
| 观测分子量 | 80 kDa |
| GenBank蛋白编号 | BC021289 |
| 基因名称 | ALPL |
| Gene ID (NCBI) | 249 |
| RRID | AB_3670648 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P05186 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ALPL antibody 82915-2-RR | Download protocol |
| FC protocol for ALPL antibody 82915-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |