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ALPL Recombinant antibody

ALPL Recombinant Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

230212D5

Cat No : 82915-2-RR

Print datasheet

Synonyms

ALPL, AP TNAP, FLJ40094, FLJ93059, HOPS, TNAP, TNSALP

验证数据展示

  • WB analysis of L02 using 82915-2-RR

    WB analysis of L02 using 82915-2-RR

    L02 cells were subjected to SDS PAGE followed by western blot with 82915-2-RR (ALPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 82915-2-RR

    FC experiment of HeLa using 82915-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ALPL Recombinant antibody (82915-2-RR, Clone:230212D5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inL02 cells
Positive FC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 82915-2-RR is ALPL Fusion Protein expressed in E. coli.

Tested Applications WB, FC, ELISA Application Description
Tested Reactivity Human
Immunogen ALPL fusion protein Ag1664 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name alkaline phosphatase, liver/bone/kidney
Synonyms ALPL, AP TNAP, FLJ40094, FLJ93059, HOPS, TNAP, TNSALP
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 80 kDa
GenBank Accession NumberBC021289
Gene Symbol ALPL
Gene ID (NCBI) 249
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP05186
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ALPL antibody 82915-2-RRDownload protocol
FC protocol for ALPL antibody 82915-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols