ALPL Recombinant antibody, PBS Only

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, FC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230212D5

Cat No : 82915-2-PBS

Synonyms

ALPL, AP TNAP, FLJ40094, FLJ93059, HOPS, TNAP, TNSALP

WB analysis of L02 using 82915-2-RR (same clone as 82915-2-PBS)
L02 cells were subjected to SDS PAGE followed by western blot with 82915-2-RR (ALPL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82915-2-PBS in a different storage buffer formulation.
FC experiment of HeLa using 82915-2-RR (same clone as 82915-2-PBS)
1x10^6 HeLa cells were intracellularly stained with 0.25 ug ALPL Recombinant antibody (82915-2-RR, Clone:230212D5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82915-2-PBS in a different storage buffer formulation.
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1x10^6 HeLa cells were intracellularly stained with 0.25 ug ALPL Recombinant antibody (82915-2-RR, Clone:230212D5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82915-2-PBS in a different storage buffer formulation.

Size

The immunogen of 82915-2-PBS is ALPL Fusion Protein expressed in E. coli.

Tested Applications	WB, FC, Indirect ELISA Application Description
Tested Reactivity	Human
Immunogen	ALPL fusion protein Ag1664 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Full Name	alkaline phosphatase, liver/bone/kidney
Synonyms	ALPL, AP TNAP, FLJ40094, FLJ93059, HOPS, TNAP, TNSALP
Calculated Molecular Weight	57 kDa
GenBank Accession Number	BC021289
Gene Symbol	ALPL
Gene ID (NCBI)	249
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purfication
UNIPROT ID	P05186
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.