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AMPK Beta 2 Monoclonal antibody, PBS Only

AMPK Beta 2 Monoclonal Antibody for WB,Indirect ELISA,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB,Indirect ELISA,IF

Conjugate

Unconjugated

CloneNo.

2D5C1

Cat No : 66579-1-PBS

Print datasheet

Synonyms

AMPK subunit beta 2, PRKAB2

验证数据展示

  • WB analysis using 66579-1-Ig (same clone as 66579-1-PBS)

    WB analysis using 66579-1-Ig (same clone as 66579-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66579-1-Ig (AMPK beta 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66579-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66579-1-Ig (same clone as 66579-1-PBS)

    IF Staining of HeLa using 66579-1-Ig (same clone as 66579-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AMPK Beta 2 antibody (66579-1-Ig, Clone: 2D5C1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66579-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy PRKAB2 Ready-To-Use IHC Kit. PRKAB2 primary antibody included.

产品信息

66579-1-PBS targets AMPK Beta 2 in WB, IF, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IF Application Description
Tested Reactivity Human, mouse, rat
Immunogen AMPK Beta 2 fusion protein Ag5806 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name protein kinase, AMP-activated, beta 2 non-catalytic subunit
Synonyms AMPK subunit beta 2, PRKAB2
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC053610
Gene Symbol PRKAB2
Gene ID (NCBI) 5565
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43741
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

AMPK beta 2 is a regulatory subunit of the AMP-activated protein kinase (AMPK), which is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK beta 2 may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles.