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验证数据展示

  • WB analysis using 66579-1-Ig

    WB analysis using 66579-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66579-1-Ig (AMPK beta 2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 66579-1-Ig

    IF Staining of HeLa using 66579-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AMPK Beta 2 antibody (66579-1-Ig, Clone: 2D5C1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

AMPK Beta 2 Monoclonal antibody

AMPK Beta 2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66579-1-Ig

产品说明书

CloneNo. 2D5C1

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

AMPK subunit beta 2, PRKAB2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA375 cells, A431 cells, HeLa cells, A549 cells, human brain tissue, rat brain tissue, mouse brain tissue
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PRKAB2 Ready-To-Use IHC Kit. PRKAB2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66579-1-Ig targets AMPK Beta 2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 AMPK Beta 2 fusion protein Ag5806 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 protein kinase, AMP-activated, beta 2 non-catalytic subunit
别名 AMPK subunit beta 2, PRKAB2
计算分子量 30 kDa
观测分子量 33 kDa
GenBank蛋白编号BC053610
基因名称 AMPK Beta 2
Gene ID (NCBI) 5565
RRIDAB_2881939
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO43741
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AMPK beta 2 is a regulatory subunit of the AMP-activated protein kinase (AMPK), which is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK beta 2 may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles.

实验方案

Product Specific Protocols
WB protocol for AMPK Beta 2 antibody 66579-1-IgDownload protocol
IF protocol for AMPK Beta 2 antibody 66579-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols