CoraLite®594-conjugated AMPK Beta 2 Monoclonal antibody
AMPK Beta 2 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
2D5C1
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy PRKAB2 Ready-To-Use IHC Kit. PRKAB2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66579-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66579 targets AMPK Beta 2 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | Human, mouse, rat |
| Immunogen | AMPK Beta 2 fusion protein Ag5806 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | protein kinase, AMP-activated, beta 2 non-catalytic subunit |
| Synonyms | AMPK subunit beta 2, PRKAB2 |
| Calculated Molecular Weight | 30 kDa |
| Observed Molecular Weight | 33 kDa |
| GenBank Accession Number | BC053610 |
| Gene Symbol | AMPK Beta 2 |
| Gene ID (NCBI) | 5565 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O43741 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AMPK beta 2 is a regulatory subunit of the AMP-activated protein kinase (AMPK), which is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK beta 2 may be a positive regulator of AMPK activity. It is highly expressed in skeletal muscle and thus may have tissue-specific roles.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 AMPK Beta 2 antibody CL594-66579 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |