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验证数据展示

  • IF Staining of HepG2 using CL488-66830

    IF Staining of HepG2 using CL488-66830

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 APOE antibody (CL488-66830, Clone: 1B2C9 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-66830

    FC experiment of HepG2 using CL488-66830

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human APOE (CL488-66830, Clone:1B2C9) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated APOE Monoclonal antibody

APOE Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66830

产品说明书

CloneNo. 1B2C9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

ApolipoproteinE, apolipoprotein E, apoe4, Apo-E, Apo E

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66830-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66830 targets APOE in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 APOE fusion protein Ag28186 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 Apolipoprotein E
别名 ApolipoproteinE, apolipoprotein E, apoe4, Apo-E, Apo E
计算分子量 36 kDa
观测分子量 34-36 kDa
GenBank蛋白编号BC003557
基因名称 APOE
Gene ID (NCBI) 348
RRIDAB_2919377
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP02649
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serve as a ligand for the LDL (APO B/E) receptor and for the specific APOE receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 APOE antibody CL488-66830Download protocol
FC protocol for CL Plus 488 APOE antibody CL488-66830Download protocol
Standard Protocols
Click here to view our Standard Protocols