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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated APOE Monoclonal antibody

APOE Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1B2C9

Cat no : CL594-66830

Print datasheet

Synonyms

ApolipoproteinE, apolipoprotein E, apoe4, Apo-E, Apo E

验证数据展示

  • IF Staining of HepG2 using CL594-66830

    IF Staining of HepG2 using CL594-66830

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 APOE antibody (CL594-66830, Clone: 1B2C9 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL594-66830

    FC experiment of HepG2 using CL594-66830

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human APOE (CL594-66830, Clone:1B2C9) (red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66830-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66830 targets APOE in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen APOE fusion protein Ag28186 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Apolipoprotein E
Synonyms ApolipoproteinE, apolipoprotein E, apoe4, Apo-E, Apo E
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 34-36 kDa
GenBank Accession NumberBC003557
Gene Symbol APOE
Gene ID (NCBI) 348
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP02649
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The apolipoprotein E (APOE) is a 299-amino acid polypeptide that mediates the binding, internalization, and catabolism of lipoprotein particles, and also serve as a ligand for the LDL (APO B/E) receptor and for the specific APOE receptor (chylomicron remnant) of hepatic tissues. The very strong association of the APOE ɛ4 allele with AD risk and its role in the accumulation of amyloid β in brains of people and animal models solidify the biological relevance of APOE isoforms but do not provide mechanistic insight.

实验方案

Product Specific Protocols
IF protocol for CL594 APOE antibody CL594-66830Download protocol
FC protocol for CL594 APOE antibody CL594-66830Download protocol
Standard Protocols
Click here to view our Standard Protocols