验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HEK-293 cells, NCCIT cell |
| Positive IF-Fro detected in | mouse heart tissue |
| Positive IF/ICC detected in | hTERT-RPE1 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66739-1-Ig targets ARL13B in WB, IHC, IF/ICC, IF-Fro, ELISA applications and shows reactivity with human, mouse, pig, canine samples.
| 经测试应用 | WB, IF/ICC, IF-Fro, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, pig, canine |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | ARL13B fusion protein Ag12031 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ADP-ribosylation factor-like 13B |
| 别名 | ARL2-like protein 1, ARL2L1, ARL2 like protein 1, ADP-ribosylation factor-like protein 2-like 1, ADP-ribosylation factor-like protein 13B |
| 计算分子量 | 48 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC094725 |
| 基因名称 | ARL13B |
| Gene ID (NCBI) | 200894 |
| RRID | AB_2882088 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q3SXY8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ARL13B antibody 66739-1-Ig | Download protocol |
| IF protocol for ARL13B antibody 66739-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex | ||
Sci Rep Targeted exon skipping rescues ciliary protein composition defects in Joubert syndrome patient fibroblasts. | ||
eNeuro CCP1, a Tubulin Deglutamylase, Increases Survival of Rodent Spinal Cord Neurons following Glutamate-Induced Excitotoxicity. | ||
Life Sci Alliance HTR6 and SSTR3 ciliary targeting relies on both IC3 loops and C-terminal tails. |
