CoraLite® Plus 647-conjugated ARL13B Monoclonal antibody
ARL13B Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2a
Reactivity
Human, Canine
Applications
IF/ICC
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
1H6C3
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | hTERT-RPE1 cells, MDCK cells |
For other applications, we recommend the unconjugated version of this antibody, 66739-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-66739 targets ARL13B in IF/ICC applications and shows reactivity with Human, Canine samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | Human, Canine |
| Immunogen | ARL13B fusion protein Ag12031 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | ADP-ribosylation factor-like 13B |
| Synonyms | ARL13B, ARL2 like protein 1, ARL2L1, DKFZp686E2075, DKFZp686L2472, DKFZp686M2074, DKFZp761H079, JBTS8 |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC094725 |
| Gene Symbol | ARL13B |
| Gene ID (NCBI) | 200894 |
| RRID | AB_2920288 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q3SXY8 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ARL13B, also named as ARL2L1, is a small ciliary G protein of the Ras superfamily. Localized in the cilia, it is required for cilium biogenesis and sonic hedgehog signaling. Defects in ARL13B are the cause of Joubert syndrome (JS) which is an autosomal recessive disorder characterized by a distinctive cerebellar malformation (PMID: 19906870). Arl13b is predicted to be a 48 kDa protein, and the 60 kDa band is likely to represent a modified form of Arl13b. ARL13B can be used to mark the cilia (PMID:22072986).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 647 ARL13B antibody CL647-66739 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
