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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84650-4-RR (same clone as 84650-4-PBS)

    WB analysis using 84650-4-RR (same clone as 84650-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84650-4-RR (ARNT,HIF1B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84650-4-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84650-4-RR (same clone as 84650-4-PBS)

    IF Staining of HepG2 using 84650-4-RR (same clone as 84650-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ARNT,HIF1B antibody (84650-4-RR, Clone: 242111G2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84650-4-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84650-4-RR (same clone as 84650-4-PBS)

    FC experiment of HeLa using 84650-4-RR (same clone as 84650-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ARNT/HIF-1 beta Recombinant antibody (84650-4-RR, Clone:242111G2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84650-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84650-4-RR

    Affinity and Kinetic Characterization of 84650-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84650-4-RR against Human ARNT/HIF-1 beta were performed. The affinity constant is 0.109 nM.

ARNT/HIF-1 beta Recombinant antibody, PBS Only

ARNT/HIF-1 beta Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84650-4-PBS

产品说明书

CloneNo. 242111G2

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

ARNT, HIF-1 beta, HIF1B, 242111G2, HIF1 beta

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ARNT Ready-To-Use IHC Kit. ARNT primary antibody included.

产品信息

84650-4-PBS targets ARNT/HIF-1 beta in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 ARNT/HIF-1 beta fusion protein Ag5507 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 aryl hydrocarbon receptor nuclear translocator
别名 ARNT, HIF-1 beta, HIF1B, 242111G2, HIF1 beta
计算分子量 416 aa, 46 kDa, 87 kDa
观测分子量 87 kDa
GenBank蛋白编号BC060838
基因名称 ARNT/HIF-1 beta
Gene ID (NCBI) 405
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP27540
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HIF1B(HIF-1 beta), also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.