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ARNT/HIF-1 beta Recombinant antibody, PBS Only

ARNT/HIF-1 beta Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242111G2

Cat no : 84650-4-PBS

Print datasheet

Synonyms

ARNT, HIF-1 beta, HIF1B, 242111G2, HIF1 beta

验证数据展示

  • WB analysis using 84650-4-RR (same clone as 84650-4-PBS)

    WB analysis using 84650-4-RR (same clone as 84650-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84650-4-RR (ARNT,HIF1B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84650-4-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84650-4-RR (same clone as 84650-4-PBS)

    IF Staining of HepG2 using 84650-4-RR (same clone as 84650-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ARNT,HIF1B antibody (84650-4-RR, Clone: 242111G2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84650-4-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84650-4-RR (same clone as 84650-4-PBS)

    FC experiment of HeLa using 84650-4-RR (same clone as 84650-4-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ARNT/HIF-1 beta Recombinant antibody (84650-4-RR, Clone:242111G2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84650-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84650-4-RR

    Affinity and Kinetic Characterization of 84650-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84650-4-RR against Human ARNT/HIF-1 beta were performed. The affinity constant is 0.109 nM.

Planning an IHC experiment? We recommend our IHCeasy ARNT Ready-To-Use IHC Kit. ARNT primary antibody included.

产品信息

84650-4-PBS targets ARNT/HIF-1 beta in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, rat
Immunogen ARNT/HIF-1 beta fusion protein Ag5507 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name aryl hydrocarbon receptor nuclear translocator
Synonyms ARNT, HIF-1 beta, HIF1B, 242111G2, HIF1 beta
Calculated Molecular Weight 416 aa, 46 kDa, 87 kDa
Observed Molecular Weight 87 kDa
GenBank Accession NumberBC060838
Gene Symbol ARNT/HIF-1 beta
Gene ID (NCBI) 405
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP27540
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HIF1B(HIF-1 beta), also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.