验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HSC-T6 cells, HeLa cells, HepG2 cells |
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | HeLa cells, HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:8000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84650-4-RR targets ARNT/HIF-1 beta in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.
经测试应用 | WB, IF/ICC, FC (Intra), ELISA Application Description |
经测试反应性 | human, rat |
免疫原 | ARNT/HIF-1 beta fusion protein Ag5507 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | aryl hydrocarbon receptor nuclear translocator |
别名 | ARNT, HIF-1 beta, HIF1B, 242111G2, HIF1 beta |
计算分子量 | 416 aa, 46 kDa, 87 kDa |
观测分子量 | 87 kDa |
GenBank蛋白编号 | BC060838 |
基因名称 | ARNT/HIF-1 beta |
Gene ID (NCBI) | 405 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | P27540 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HIF1B(HIF-1 beta), also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.
实验方案
Product Specific Protocols | |
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WB protocol for ARNT/HIF-1 beta antibody 84650-4-RR | Download protocol |
IF protocol for ARNT/HIF-1 beta antibody 84650-4-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |