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ARNT/HIF-1 beta Recombinant antibody

ARNT/HIF-1 beta Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

242111G2

Cat no : 84650-4-RR

Print datasheet

Synonyms

ARNT, HIF-1 beta, HIF1B, 242111G2, HIF1 beta

验证数据展示

  • WB analysis using 84650-4-RR

    WB analysis using 84650-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84650-4-RR (ARNT,HIF1B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 84650-4-RR

    IF Staining of HepG2 using 84650-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ARNT,HIF1B antibody (84650-4-RR, Clone: 242111G2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 84650-4-RR

    FC experiment of HeLa using 84650-4-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ARNT/HIF-1 beta Recombinant antibody (84650-4-RR, Clone:242111G2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 84650-4-RR

    Affinity and Kinetic Characterization of 84650-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84650-4-RR against Human ARNT/HIF-1 beta were performed. The affinity constant is 0.109 nM.

经过测试的应用

Positive WB detected inA549 cells, HSC-T6 cells, HeLa cells, HepG2 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ARNT Ready-To-Use IHC Kit. ARNT primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84650-4-RR targets ARNT/HIF-1 beta in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, rat
Immunogen ARNT/HIF-1 beta fusion protein Ag5507 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name aryl hydrocarbon receptor nuclear translocator
Synonyms ARNT, HIF-1 beta, HIF1B, 242111G2, HIF1 beta
Calculated Molecular Weight 416 aa, 46 kDa, 87 kDa
Observed Molecular Weight 87 kDa
GenBank Accession NumberBC060838
Gene Symbol ARNT/HIF-1 beta
Gene ID (NCBI) 405
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP27540
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HIF1B(HIF-1 beta), also named as ARNT, is a 789 amino acid protein, which forms a heterodimer with AHR, AHRR, HIF1A and EPAS1/HIF2A as well as with other bHLH proteins. HIF1B is Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.

实验方案

Product Specific Protocols
WB protocol for ARNT/HIF-1 beta antibody 84650-4-RRDownload protocol
IF protocol for ARNT/HIF-1 beta antibody 84650-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols