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CoraLite® Plus 488-conjugated ASCC2 Polyclonal antibody

ASCC2 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11529

Print datasheet

Synonyms

RQT3, ASC1P100, ASC-1 complex subunit p100

验证数据展示

  • FC experiment of HepG2 using CL488-11529

    FC experiment of HepG2 using CL488-11529

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Ascc2 Polyclonal Antibody (CL488-11529)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL488-11529

    IF Staining of HepG2 using CL488-11529

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 ASCC2 antibody (CL488-11529) at dilution of 1:200, CL594-Phalloidin (red).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 11529-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11529 targets ASCC2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen ASCC2 fusion protein Ag2100 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name activating signal cointegrator 1 complex subunit 2
Synonyms RQT3, ASC1P100, ASC-1 complex subunit p100
Calculated Molecular Weight 757 aa, 86 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC025368
Gene Symbol ASCC2
Gene ID (NCBI) 84164
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H1I8
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ASCC2 antibody CL488-11529Download protocol
FC protocol for CL Plus 488 ASCC2 antibody CL488-11529Download protocol
Standard Protocols
Click here to view our Standard Protocols