• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of HepG2 using CL488-66671

    IF Staining of HepG2 using CL488-66671

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488-conjugated ASF/SF2 antibody (CL488-66671, Clone: 1A6C5 ) at dilution of 1:100.

CoraLite® Plus 488-conjugated ASF/SF2 Monoclonal antibody

ASF/SF2 Monoclonal Antibody for IF/ICC
Cat No. CL488-66671

产品说明书

CloneNo. 1A6C5

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

IF/ICC

别名

Alternative splicing factor 1, ASF, ASF 1, ASF/SF2, SF2, SF2p33, SFRS1, SRp30a, SRSF1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SRSF1 Ready-To-Use IHC Kit. SRSF1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66671-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66671 targets ASF/SF2 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, mouse, rat
免疫原 ASF/SF2 fusion protein Ag3626 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 splicing factor, arginine/serine-rich 1
别名 Alternative splicing factor 1, ASF, ASF 1, ASF/SF2, SF2, SF2p33, SFRS1, SRp30a, SRSF1
计算分子量 248 aa, 28 kDa
观测分子量 32 kDa, 28 kDa, 22 kDa
GenBank蛋白编号BC010264
基因名称 ASF/SF2
Gene ID (NCBI) 6426
RRIDAB_2919354
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ07955
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SFRS1, also named as ASF, SF2, SF2P33, SFRS1 and ASF-1, belongs to the splicing factor SR family. It plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. SFRS1 interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. This antibody can recognize all the 3 isoforms(28kd,33kd and 22kd) of SFRS1.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ASF/SF2 antibody CL488-66671Download protocol
Standard Protocols
Click here to view our Standard Protocols