CoraLite®594-conjugated ASF/SF2 Monoclonal antibody
ASF/SF2 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1A6C5
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy SRSF1 Ready-To-Use IHC Kit. SRSF1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66671-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66671 targets ASF/SF2 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | Human, mouse, rat |
| Immunogen | ASF/SF2 fusion protein Ag3626 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | splicing factor, arginine/serine-rich 1 |
| Synonyms | Alternative splicing factor 1, ASF, ASF 1, ASF/SF2, SF2, SF2p33, SFRS1, SRp30a, SRSF1 |
| Calculated Molecular Weight | 248 aa, 28 kDa |
| Observed Molecular Weight | 32 kDa, 28 kDa, 22 kDa |
| GenBank Accession Number | BC010264 |
| Gene Symbol | ASF/SF2 |
| Gene ID (NCBI) | 6426 |
| RRID | AB_2920019 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q07955 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SFRS1, also named as ASF, SF2, SF2P33, SFRS1 and ASF-1, belongs to the splicing factor SR family. It plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. SFRS1 interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. This antibody can recognize all the 3 isoforms(28kd,33kd and 22kd) of SFRS1.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 ASF/SF2 antibody CL594-66671 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |