CoraLite®594-conjugated ASF/SF2 Monoclonal antibody

ASF/SF2 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A6C5

Cat No : CL594-66671

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Synonyms

Alternative splicing factor 1, ASF, ASF 1, ASF/SF2, SF2, SF2p33, SFRS1, SRp30a, SRSF1



经过测试的应用

Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SRSF1 Ready-To-Use IHC Kit. SRSF1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66671-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66671 targets ASF/SF2 in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF
Tested Reactivity Human, mouse, rat
Immunogen ASF/SF2 fusion protein Ag3626 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name splicing factor, arginine/serine-rich 1
Synonyms Alternative splicing factor 1, ASF, ASF 1, ASF/SF2, SF2, SF2p33, SFRS1, SRp30a, SRSF1
Calculated Molecular Weight 248 aa, 28 kDa
Observed Molecular Weight 32 kDa, 28 kDa, 22 kDa
GenBank Accession NumberBC010264
Gene Symbol SFRS1
Gene ID (NCBI) 6426
RRIDAB_2920019
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ07955
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

SFRS1, also named as ASF, SF2, SF2P33, SFRS1 and ASF-1, belongs to the splicing factor SR family. It plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. SFRS1 interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. This antibody can recognize all the 3 isoforms(28kd,33kd and 22kd) of SFRS1.

实验方案

Product Specific Protocols
IF protocol for CL594 ASF/SF2 antibody CL594-66671Download protocol
Standard Protocols
Click here to view our Standard Protocols