ASL Monoclonal antibody
ASL Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, mouse, rat, pig
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1G9H3
验证数据展示
经过测试的应用
| Positive WB detected in | pig kidney tissue, LNCaP cells, K-562 cells, rat kidney tissue, mouse kidney tissue, pig liver tissue, rat liver tissue, mouse liver tissue |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
67692-1-Ig targets ASL in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | Human, mouse, rat, pig |
| Cited Reactivity | mouse |
| Immunogen | ASL fusion protein Ag30517 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | argininosuccinate lyase |
| Synonyms | argininosuccinate lyase, Arginosuccinase, ASAL, ASL |
| Calculated Molecular Weight | 55 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC008195 |
| Gene Symbol | ASL |
| Gene ID (NCBI) | 435 |
| RRID | AB_2882885 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P04424 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Argininosuccinate lyase (ASL), one of the significant UC enzymes, catalyzes argininosuccinate cleavage to generate arginine and fumarate. Arginine is then catalyzed by arginase to ornithine and polyamines, which are found to promote cancer cell proliferation and growth. Importantly, ASL ectopic expression is closely associated with poor prognosis of colorectal cancer, hepatocellular. ASL is a member of the aspartase/fumarase superfamily. Enzymes of this superfamily share similar tetrameric structure and active site, though the sequence identities between different members are quite low (less than 20%). Members of this superfamily have been recognised as drug targets for microbial infections. ASL is a 464-amino-acid enzyme with a molecular mass of 49.7 kDa. (PMID: 22386318 PMID: 31018905)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ASL antibody 67692-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO Mol Med A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy |