验证数据展示
经过测试的应用
| Positive WB detected in | pig kidney tissue, LNCaP cells, K-562 cells, rat kidney tissue, mouse kidney tissue, pig liver tissue, rat liver tissue, mouse liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
67692-1-Ig targets ASL in WB, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, mouse, rat, pig |
| 文献引用反应性 | mouse |
| 免疫原 | ASL fusion protein Ag30517 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | argininosuccinate lyase |
| 别名 | argininosuccinate lyase, Arginosuccinase, ASAL, ASL |
| 计算分子量 | 55 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC008195 |
| 基因名称 | ASL |
| Gene ID (NCBI) | 435 |
| RRID | AB_2882885 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P04424 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Argininosuccinate lyase (ASL), one of the significant UC enzymes, catalyzes argininosuccinate cleavage to generate arginine and fumarate. Arginine is then catalyzed by arginase to ornithine and polyamines, which are found to promote cancer cell proliferation and growth. Importantly, ASL ectopic expression is closely associated with poor prognosis of colorectal cancer, hepatocellular. ASL is a member of the aspartase/fumarase superfamily. Enzymes of this superfamily share similar tetrameric structure and active site, though the sequence identities between different members are quite low (less than 20%). Members of this superfamily have been recognised as drug targets for microbial infections. ASL is a 464-amino-acid enzyme with a molecular mass of 49.7 kDa. (PMID: 22386318 PMID: 31018905)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ASL antibody 67692-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |