验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | K-562 cells |
| Positive FC detected in | K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67692 targets ASL in FC (Intra) applications and shows reactivity with Human, mouse, rat, pig samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | Human, mouse, rat, pig |
| 免疫原 | ASL fusion protein Ag30517 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | argininosuccinate lyase |
| 别名 | argininosuccinate lyase, Arginosuccinase, ASAL, ASL |
| 计算分子量 | 55 kDa |
| 观测分子量 | 50 kDa |
| GenBank蛋白编号 | BC008195 |
| 基因名称 | ASL |
| Gene ID (NCBI) | 435 |
| RRID | AB_2923781 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P04424 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Argininosuccinate lyase (ASL), one of the significant UC enzymes, catalyzes argininosuccinate cleavage to generate arginine and fumarate. Arginine is then catalyzed by arginase to ornithine and polyamines, which are found to promote cancer cell proliferation and growth. Importantly, ASL ectopic expression is closely associated with poor prognosis of colorectal cancer, hepatocellular. ASL is a member of the aspartase/fumarase superfamily. Enzymes of this superfamily share similar tetrameric structure and active site, though the sequence identities between different members are quite low (less than 20%). Members of this superfamily have been recognised as drug targets for microbial infections. ASL is a 464-amino-acid enzyme with a molecular mass of 49.7 kDa. (PMID: 22386318 PMID: 31018905)
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 ASL antibody CL488-67692 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |