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验证数据展示

  • IF Staining of HeLa using CL488-66346

    IF Staining of HeLa using CL488-66346

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 ASNA1 antibody (CL488-66346, Clone: 1B7C11 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated ASNA1 Monoclonal antibody

ASNA1 Monoclonal Antibody for IF/ICC
Cat No. CL488-66346

产品说明书

CloneNo. 1B7C11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

IF/ICC

别名

TRC40, ATPase GET3, ASNA 1, Arsenite-stimulated ATPase, Arsenical pump-driving ATPase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66346-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66346 targets ASNA1 in IF/ICC applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat, pig
免疫原 ASNA1 fusion protein Ag7936 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)
别名 TRC40, ATPase GET3, ASNA 1, Arsenite-stimulated ATPase, Arsenical pump-driving ATPase
计算分子量 38.7 kDa
观测分子量 37-41 kDa
GenBank蛋白编号BC002651
基因名称 ASNA1
Gene ID (NCBI) 439
RRIDAB_2883314
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO43681
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ASNA1 (also known as TRC40) is a highly conserved ATPase involved in efflux of arsenite and antimonite. Reduced ASNA1 expression is associated with significant inhibition of cell growth, increased apoptosis and increased sensitivity to arsenite.Thus ASNA1 is proposed to be a target to overcome resistance to cancer chemotherapy. In addition, ASNA1 has been identified as an ER targeting factor for tail-anchored proteins in the posttranslational membrane insertion pathway.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ASNA1 antibody CL488-66346Download protocol
Standard Protocols
Click here to view our Standard Protocols