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CoraLite®594-conjugated ASNA1 Monoclonal antibody

ASNA1 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1B7C11

Cat No : CL594-66346

Print datasheet

Synonyms

TRC40, ATPase GET3, ASNA 1, Arsenite-stimulated ATPase, Arsenical pump-driving ATPase

验证数据展示

  • IF Staining of HEK-293 using CL594-66346

    IF Staining of HEK-293 using CL594-66346

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CL594-66346 (ASNA1 antibody) at dilution of 1:100.

  • FC experiment of HEK-293 using CL594-66346

    FC experiment of HEK-293 using CL594-66346

    1X10^6 HEK-293 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human ASNA1 (CL594-66346, Clone:1B7C11) (red), or 0.8 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 66346-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66346 targets ASNA1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen ASNA1 fusion protein Ag7936 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)
Synonyms TRC40, ATPase GET3, ASNA 1, Arsenite-stimulated ATPase, Arsenical pump-driving ATPase
Calculated Molecular Weight 38.7 kDa
Observed Molecular Weight 37-41 kDa
GenBank Accession NumberBC002651
Gene Symbol ASNA1
Gene ID (NCBI) 439
RRIDAB_2883564
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43681
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ASNA1 (also known as TRC40) is a highly conserved ATPase involved in efflux of arsenite and antimonite. Reduced ASNA1 expression is associated with significant inhibition of cell growth, increased apoptosis and increased sensitivity to arsenite.Thus ASNA1 is proposed to be a target to overcome resistance to cancer chemotherapy. In addition, ASNA1 has been identified as an ER targeting factor for tail-anchored proteins in the posttranslational membrane insertion pathway.

实验方案

Product Specific Protocols
IF protocol for CL594 ASNA1 antibody CL594-66346Download protocol
Standard Protocols
Click here to view our Standard Protocols