- Featured Product
- KD/KO Validated
ASS1 Polyclonal antibody
ASS1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, rat kidney tissue, SMMC-7721 cells, Jurkat cells, mouse kidney tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human testis tissue, human liver tissue, human placenta tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy ASS1 Ready-To-Use IHC Kit. ASS1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16210-1-AP targets ASS1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | ASS1 fusion protein Ag9200 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | argininosuccinate synthetase 1 |
| Synonyms | EC:6.3.4.5, ASS |
| Calculated Molecular Weight | 412 aa, 47 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC009243 |
| Gene Symbol | ASS1 |
| Gene ID (NCBI) | 445 |
| RRID | AB_2060466 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P00966 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ASS1 is a rate-limiting enzyme in the urea cycle. Citrullinemia type I is an autosomal recessive disorder that is caused by a deficiency of the urea cycle enzyme argininosuccinate synthetase (ASS1). Deficiency of ASS1 shows various clinical manifestations encompassing severely affected patients with fatal neonatal hyperammonemia as well as asymptomatic individuals with only a biochemical phenotype(PMID:19006241).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ASS1 antibody 16210-1-AP | Download protocol |
| IHC protocol for ASS1 antibody 16210-1-AP | Download protocol |
| IF protocol for ASS1 antibody 16210-1-AP | Download protocol |
| IP protocol for ASS1 antibody 16210-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Immunity Excessive Polyamine Generation in Keratinocytes Promotes Self-RNA Sensing by Dendritic Cells in Psoriasis. | ||
Sci Transl Med An extracorporeal bioartificial liver embedded with 3D-layered human liver progenitor-like cells relieves acute liver failure in pigs. | ||
Mol Cell Arginine deprivation enriches lung cancer proteomes with cysteine by inducing arginine-to-cysteine substitutants | ||
Autophagy BNIP3-dependent mitophagy promotes cytosolic localization of LC3B and metabolic homeostasis in the liver. | ||
J Am Soc Nephrol Hnf4a Is Required for the Development of Cdh6-Expressing Progenitors into Proximal Tubules in the Mouse Kidney. |
