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ASNS Polyclonal antibody
ASNS Polyclonal Antibody for IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
IHC, IF/ICC, IP, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human retinoblastoma tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | K-562 cells |
Planning an IHC experiment? We recommend our IHCeasy ASNS Ready-To-Use IHC Kit. ASNS primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14681-1-AP targets ASNS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Applications | IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Immunogen | ASNS fusion protein Ag6393 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | asparagine synthetase |
| Synonyms | TS11, Glutamine-dependent asparagine synthetase, EC:6.3.5.4, Asparagine synthetase [glutamine-hydrolyzing] |
| Calculated Molecular Weight | 64 kDa |
| Observed Molecular Weight | 55-64 kDa |
| GenBank Accession Number | BC014621 |
| Gene Symbol | ASNS |
| Gene ID (NCBI) | 440 |
| RRID | AB_2060119 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P08243 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Asparagine synthetase [glutamine-hydrolyzing](ASNS) is also named as TS11. The asparagine synthetase gene, encoding the enzyme that catalyzes the synthesis of asparagine and glutamate using glutamine and aspartate, is a gene for which transcription is highly regulated by the nutritional status of the cell(PMID:15385533). ASNS has some isoforms with the MW of 55-64 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ASNS antibody 14681-1-AP | Download protocol |
| IHC protocol for ASNS antibody 14681-1-AP | Download protocol |
| IF protocol for ASNS antibody 14681-1-AP | Download protocol |
| IP protocol for ASNS antibody 14681-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab ZBTB1 Regulates Asparagine Synthesis and Leukemia Cell Response to L-Asparaginase. | ||
Cell Metab Asparagine couples mitochondrial respiration to ATF4 activity and tumor growth.
| ||
Mol Cell Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.
| ||
Mol Cell Asparagine plays a critical role in regulating cellular adaptation to glutamine depletion.
| ||
Nat Commun p53-mediated control of aspartate-asparagine homeostasis dictates LKB1 activity and modulates cell survival.
| ||
J Exp Med Glutamine depletion regulates Slug to promote EMT and metastasis in pancreatic cancer. |
